WB (Western Blot)
(Drosha antibody (pAb) tested by Western blot. HeLa cell nuclear extract (40 ug) probed with Drosha antibody at a dilution of 1:500.)
Rabbit anti-Human Drosha Polyclonal Antibody | anti-Drosha antibody
Drosha antibody (pAb)
Gene Names
DROSHA; RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Affinity Purified
Synonyms
Drosha, Antibody; Drosha antibody (pAb); RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976; anti-Drosha antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
0.44ug/ul (varies by lot)
Applicable Applications for anti-Drosha antibody
WB (Western Blot), IP (Immunoprecipitation)
Immunogen
This Drosha antibody was raised against a peptide within the C-terminal region of human Drosha.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(Drosha antibody (pAb) tested by Western blot. HeLa cell nuclear extract (40 ug) probed with Drosha antibody at a dilution of 1:500.)
WB (Western Blot)
(Drosha antibody (pAb) tested by Western blot. HeLa cell nuclear extract (40 ug) probed with Drosha antibody at a dilution of 1:500.)
WB (Western Blot)
(Drosha antibody (pAb) tested by Immunoprecipitation. 10 ul of Drosha antibody was used to immunoprecipitate Drosha from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the Drosha antibody at a dilution of 1:500.)
WB (Western Blot)
(Drosha antibody (pAb) tested by Immunoprecipitation. 10 ul of Drosha antibody was used to immunoprecipitate Drosha from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the Drosha antibody at a dilution of 1:500.)
Related Product Information for anti-Drosha antibody
Short Description: Our Drosha antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: Drosha is an RNAse III enzyme that is involved in the process of generating microRNA (miRNA). miRNA interacts with the RNA-induced silencing complex (RISC) to cleave mRNA and thus downregulate gene expression. Drosha is a double stranded RNAse and part of the Microprocessor complex. Other RNAse III enzymes include Dicer and Argonaute. Drosha initiates processing of the miRNA precursor (pri-miRNA) in the nucleus, which is then exported to the cytoplasm and further cleaved by Dicer into a mature miRNA.
Background: Drosha is an RNAse III enzyme that is involved in the process of generating microRNA (miRNA). miRNA interacts with the RNA-induced silencing complex (RISC) to cleave mRNA and thus downregulate gene expression. Drosha is a double stranded RNAse and part of the Microprocessor complex. Other RNAse III enzymes include Dicer and Argonaute. Drosha initiates processing of the miRNA precursor (pri-miRNA) in the nucleus, which is then exported to the cytoplasm and further cleaved by Dicer into a mature miRNA.
Product Categories/Family for anti-Drosha antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
159kDa
NCBI Official Full Name
ribonuclease 3 isoform 1
NCBI Official Synonym Full Names
drosha ribonuclease III
NCBI Official Symbol
DROSHA
NCBI Official Synonym Symbols
RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976
NCBI Protein Information
ribonuclease 3
UniProt Protein Name
Ribonuclease 3
UniProt Gene Name
DROSHA
UniProt Synonym Gene Names
RN3; RNASE3L; RNASEN; RNase III
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Product Notes
The Drosha drosha (Catalog #AAA59929) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Drosha antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Drosha can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the Drosha drosha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Drosha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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