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IHC (Immunohistochemistry)
(At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit DUSP16 Polyclonal Antibody | anti-DUSP16 antibody
Phospho-DUSP16 (Ser446) Antibody
Gene Names
DUSP16; MKP7; MKP-7
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (86%), Zebrafish (86%), Bovine (86%), Rabbit (86%), Chicken (86%)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
DUSP16, Antibody; Phospho-DUSP16 (Ser446) Antibody; Dual specificity phosphatase 16; Dual specificity protein phosphatase 16; DUS16_HUMAN; DUSP 16; DUSP16; KIAA1700; MAP kinase phosphatase 7; MAPK phosphatase 7; Mitogen activated protein kinase phosphatase 7; Mitogen-activated protein kinase phosphatase 7; MKP 7; MKP-7; MKP7; anti-DUSP16 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (86%), Zebrafish (86%), Bovine (86%), Rabbit (86%), Chicken (86%)
Predicted Reactivity: Pig (86%), Zebrafish (86%), Bovine (86%), Rabbit (86%), Chicken (86%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-DUSP16 (Ser446) Antibody detects endogenous levels of DUSP16 only when phosphorylated at Ser446.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-DUSP16 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human DUSP16 around the phosphorylation site of Ser446.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated at Ser-446 by MAPK1/ERK2, which prevents its degradation, and thereby stabilizes it and blocks JNK MAPK activity. (Microbial infection) Acetylated at Lys-55 by the M.tuberculosis Eis protein; this leads to the inhibition of JNK-dependent autophagy, phagosome maturation, and ROS (reactive oxygen species) generation for enhanced intracellular survival of M.tuberculosis.
Subunit Structure
Interacts with ARRB2.
Similarity
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Subcellular Location
Cytoplasm. Nucleus. Cytoplasmic vesicle.
Note: After dissociation upon AGTR stimulation, re-associates with ARRB2 on endocytic vesicles.
Note: After dissociation upon AGTR stimulation, re-associates with ARRB2 on endocytic vesicles.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of MKP-7 (Phospho-Ser446) using TNF-α treated A2780 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of MKP-7 (Phospho-Ser446) using TNF-α treated A2780 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Related Product Information for anti-DUSP16 antibody
Dual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73,102 Da
NCBI Official Full Name
dual specificity protein phosphatase 16
NCBI Official Synonym Full Names
dual specificity phosphatase 16
NCBI Official Symbol
DUSP16
NCBI Official Synonym Symbols
MKP7; MKP-7
NCBI Protein Information
dual specificity protein phosphatase 16; MAPK phosphatase-7; MAP kinase phosphatase 7; mitogen-activated protein kinase phosphatase 7
UniProt Protein Name
Dual specificity protein phosphatase 16
UniProt Gene Name
DUSP16
UniProt Synonym Gene Names
KIAA1700; MKP7; MAP kinase phosphatase 7; MKP-7
UniProt Entry Name
DUS16_HUMAN
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Product Notes
The DUSP16 dusp16 (Catalog #AAA329819) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-DUSP16 (Ser446) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (86%), Zebrafish (86%), Bovine (86%), Rabbit (86%), Chicken (86%) and may cross-react with other species as described in the data sheet. AAA Biotech's DUSP16 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DUSP16 dusp16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DUSP16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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