WB (Western Blot)
(Detection of human and mouse E2-25K by western blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-E2-25K antibody AAA212508 (lot AAA212508-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse E2-25K Polyclonal Antibody | anti-E2-25K antibody
Rabbit anti-E2-25K Antibody, Affinity Purified
Gene Names
UBE2K; LIG; HIP2; HYPG; UBC1; E2-25K
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
E2-25K, Antibody; Rabbit anti-E2-25K Antibody, Affinity Purified; ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-protein ligase; Ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2(25K); ubiquitin-conjugating enzyme E2 K; ubiquitin conjugating enzyme E2K; ubiquitin carrier protein; UBC1; HYPG; huntingtin-interacting protein 2; HIP-2; HIP2; E2-25K; E2(25K); E2 ubiquitin-conjugating enzyme K; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); LIG; Ubiquitin-conjugating enzyme E2 K; ubiquitin conjugating enzyme E2 K; anti-E2-25K antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-E2-25K antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 150 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse E2-25K by western blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-E2-25K antibody AAA212508 (lot AAA212508-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse E2-25K by western blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-E2-25K antibody AAA212508 (lot AAA212508-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human E2-25K by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-E2-25K antibody AAA212508 (lot AAA212508-1) used for IP at 6 ug/mg lysate. E2-25K was inefficiently immunoprecipitated by rabbit anti-E2-25K antibody BL14896. For blotting immunoprecipitated E2-25K, AAA212508 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human E2-25K by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-E2-25K antibody AAA212508 (lot AAA212508-1) used for IP at 6 ug/mg lysate. E2-25K was inefficiently immunoprecipitated by rabbit anti-E2-25K antibody BL14896. For blotting immunoprecipitated E2-25K, AAA212508 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-E2-25K antibody
Ubiquitin-conjugating enzyme E2-25 kDa (E2-25K) belongs to the ubiquitin-conjugating enzyme family. E2-25K interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation [taken from NCBI Entrez Gene (Gene ID: 3093)].
Product Categories/Family for anti-E2-25K antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,447 Da
NCBI Official Full Name
Homo sapiens ubiquitin-conjugating enzyme E2K (UBE2K), transcript variant 1, mRNA
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2K
NCBI Official Symbol
UBE2K
NCBI Official Synonym Symbols
LIG; HIP2; HYPG; UBC1; E2-25K
NCBI Protein Information
ubiquitin-conjugating enzyme E2 K; E2(25K); HIP-2; huntingtin interacting protein 2; huntingtin-interacting protein 2; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2(25K); ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-conjugating enzyme E2
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 K
UniProt Gene Name
UBE2K
UniProt Synonym Gene Names
HIP2; LIG; HIP-2; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25K
UniProt Entry Name
UBE2K_HUMAN
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Product Notes
The E2-25K ube2k (Catalog #AAA212508) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-E2-25K Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's E2-25K can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the E2-25K ube2k for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E2-25K, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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