WB (Western Blot)
(Detection of human E2F4 by western blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug) and HEK293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F4 antibody AAA211774 (lot AAA211774-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human E2F4 Polyclonal Antibody | anti-E2F4 antibody
Rabbit anti-E2F4 Antibody, Affinity Purified
Gene Names
E2F4; E2F-4
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
E2F4, Antibody; Rabbit anti-E2F4 Antibody, Affinity Purified; transcription factor E2F4; E2F transcription factor 4, p107/p130-binding; E2F-4; p107/p130-binding protein; Transcription factor E2F4; E2F transcription factor 4; anti-E2F4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-E2F4 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 363 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human E2F4 by western blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug) and HEK293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F4 antibody AAA211774 (lot AAA211774-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human E2F4 by western blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug) and HEK293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F4 antibody AAA211774 (lot AAA211774-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human E2F4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F4 antibody AAA211774 (lot AAA211774-2) used for IP at 6 ug per reaction. E2F4 was also immunoprecipitated by rabbit anti-E2F4 antibody For blotting immunoprecipitated E2F4, AAA211774 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human E2F4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F4 antibody AAA211774 (lot AAA211774-2) used for IP at 6 ug per reaction. E2F4 was also immunoprecipitated by rabbit anti-E2F4 antibody For blotting immunoprecipitated E2F4, AAA211774 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human E2F4 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-E2F4 (Cat. No. AAA211774 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human E2F4 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-E2F4 (Cat. No. AAA211774 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-E2F4 antibody
E2F4 is a member of the E2F family of transcription factors. It binds DNA at the E2 recognition site found in the promoter region of a number of genes involved in cell cycle regulation and DNA replication. E2F4 binds to pRb, p107, and p130. Via its interaction with p130, E2F4 plays an important role in maintaining G2 arrest during G2 phase checkpoint activation.
Product Categories/Family for anti-E2F4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,960 Da
NCBI Official Full Name
transcription factor E2F4
NCBI Official Synonym Full Names
E2F transcription factor 4
NCBI Official Symbol
E2F4
NCBI Official Synonym Symbols
E2F-4
NCBI Protein Information
transcription factor E2F4
UniProt Protein Name
Transcription factor E2F4
UniProt Gene Name
E2F4
UniProt Synonym Gene Names
E2F-4
UniProt Entry Name
E2F4_HUMAN
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Product Notes
The E2F4 e2f4 (Catalog #AAA211774) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-E2F4 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's E2F4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the E2F4 e2f4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E2F4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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