IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human testis using PA889102LA01HU at dilution 1:100)
Rabbit anti-Human E3 ubiquitin-protein ligase RAD18 Polyclonal Antibody | anti-RAD18 antibody
Rabbit anti-human E3 ubiquitin-protein ligase RAD18 polyclonal Antibody
Gene Names
RAD18; RNF73
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
E3 ubiquitin-protein ligase RAD18, Antibody; Rabbit anti-human E3 ubiquitin-protein ligase RAD18 polyclonal Antibody; Postreplication repair protein RAD18; RING finger protein 73; RAD18; RNF73; anti-RAD18 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
495
Applicable Applications for anti-RAD18 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human E3 ubiquitin-protein ligase RAD18 protein(1-495aa)
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-27077 / sc-98946
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human testis using PA889102LA01HU at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human testis using PA889102LA01HU at dilution 1:100)
WB (Western Blot)
(Western blotAll lanes: E3 ubiquitin-protein ligase RAD18 antibody at 2ug/ml+COLO205 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 56kDaObserved band size: 56kDa)
WB (Western Blot)
(Western blotAll lanes: E3 ubiquitin-protein ligase RAD18 antibody at 2ug/ml+COLO205 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 56kDaObserved band size: 56kDa)
Related Product Information for anti-RAD18 antibody
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on Lys-164. Has ssDNA binding activity.
This protein is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
This protein is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,223 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase RAD18
NCBI Official Synonym Full Names
RAD18, E3 ubiquitin protein ligase
NCBI Official Symbol
RAD18
NCBI Official Synonym Symbols
RNF73
NCBI Protein Information
E3 ubiquitin-protein ligase RAD18
UniProt Protein Name
E3 ubiquitin-protein ligase RAD18
UniProt Gene Name
RAD18
UniProt Synonym Gene Names
RNF73; hHR18; hRAD18
UniProt Entry Name
RAD18_HUMAN
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Product Notes
The RAD18 rad18 (Catalog #AAA229874) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human E3 ubiquitin-protein ligase RAD18 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's E3 ubiquitin-protein ligase RAD18 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the RAD18 rad18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E3 ubiquitin-protein ligase RAD18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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