WB (Western Blot)
(Detection of human and mouse EBP1 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T, 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EBP1 antibody AAA212110 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
Rabbit anti-Human, Mouse EBP1 Polyclonal Antibody | anti-EBP1 antibody
Rabbit anti-EBP1 Antibody, Affinity Purified
Gene Names
PA2G4; EBP1; HG4-1; p38-2G4
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
EBP1, Antibody; Rabbit anti-EBP1 Antibody, Affinity Purified; ErbB-3 binding protein 1; p38-2G4; proliferation-associated protein 2G4; proliferation-associated 2G4, 38kDa; proliferation-associated 2G4, 38kD; HG4-1; erbB3-binding protein 1; EBP1; cell cycle protein p38-2G4 homolog; ErbB3-binding protein Ebp1; Proliferation-associated protein 2G4; proliferation-associated 2G4; anti-EBP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-EBP1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 344 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse EBP1 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T, 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EBP1 antibody AAA212110 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human and mouse EBP1 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T, 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EBP1 antibody AAA212110 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human EBP1 by immunohistochemistry. Samples: FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EBP1 (Cat. No. Lot1, left image and Cat. No. AAA212110 Lot1, right image) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human EBP1 by immunohistochemistry. Samples: FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EBP1 (Cat. No. Lot1, left image and Cat. No. AAA212110 Lot1, right image) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
Related Product Information for anti-EBP1 antibody
ErbB-3 binding protein (EBP1) may play a role in a ERBB3-regulated signal transduction pathway. EBP1 Seems be involved in growth regulation. It acts as a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG).
Product Categories/Family for anti-EBP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,787 Da
NCBI Official Full Name
proliferation-associated protein 2G4
NCBI Official Synonym Full Names
proliferation-associated 2G4, 38kDa
NCBI Official Symbol
PA2G4
NCBI Official Synonym Symbols
EBP1; HG4-1; p38-2G4
NCBI Protein Information
proliferation-associated protein 2G4; erbB3-binding protein 1; ErbB-3 binding protein 1; ErbB3-binding protein Ebp1; cell cycle protein p38-2G4 homolog
UniProt Protein Name
Proliferation-associated protein 2G4
UniProt Gene Name
PA2G4
UniProt Synonym Gene Names
EBP1; hG4-1
UniProt Entry Name
PA2G4_HUMAN
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Product Notes
The EBP1 pa2g4 (Catalog #AAA212110) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-EBP1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EBP1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the EBP1 pa2g4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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