WB (Western Blot)
(Detection of ECS (DYKDDDDK)-tagged protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified goat anti-ECS (DYKDDDDK) antibody AAA210710 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.)
Goat ECS (DYKDDDDK) Tag Polyclonal Antibody | anti-ECS antibody
Goat anti-ECS (DYKDDDDK) Tag Antibody Affinity Purified
Applications
ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ECS (DYKDDDDK) Tag, Antibody; Goat anti-ECS (DYKDDDDK) Tag Antibody Affinity Purified; anti-ECS antibody
Host
Goat
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ECS antibody
ELISA, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of ECS (DYKDDDDK)-tagged protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified goat anti-ECS (DYKDDDDK) antibody AAA210710 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of ECS (DYKDDDDK)-tagged protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified goat anti-ECS (DYKDDDDK) antibody AAA210710 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.)
ICC (Immunocytochemistry)
(Detection of DYKDDDDK-tagged Beta-Catenin by immunofluorescence. Samples: Human microvascular endothelial cells expressing DYKDDDDK-tagged beta-catenin following transient transfection. Antibody: Affinity purified goat anti-ECS (DYKDDDDK) (Cat. No. AAA210710) used at 1 ug/ml. Detection: FITC labeled rabbit anti-goat IgG (h+l).)
ICC (Immunocytochemistry)
(Detection of DYKDDDDK-tagged Beta-Catenin by immunofluorescence. Samples: Human microvascular endothelial cells expressing DYKDDDDK-tagged beta-catenin following transient transfection. Antibody: Affinity purified goat anti-ECS (DYKDDDDK) (Cat. No. AAA210710) used at 1 ug/ml. Detection: FITC labeled rabbit anti-goat IgG (h+l).)
Related Product Information for anti-ECS antibody
The ECS epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized ECS epitope represents the enterokinase cleavage site DDDDK.
Product Categories/Family for anti-ECS antibody
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Product Notes
The ECS (Catalog #AAA210710) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ECS (DYKDDDDK) Tag can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ECS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ECS (DYKDDDDK) Tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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