WB (Western Blot)
(Detection of human EDF-1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-EDF-1 antibody AAA212467 (lot AAA212467-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human EDF-1 Polyclonal Antibody | anti-EDF-1 antibody
Rabbit anti-EDF-1 Antibody, Affinity Purified
Gene Names
EDF1; MBF1; EDF-1
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
EDF-1, Antibody; Rabbit anti-EDF-1 Antibody, Affinity Purified; Multiprotein-bridging factor 1; MBF1; endothelial differentiation-related factor 1; EDF-1; CFAP280; multiprotein bridging factor 1; Endothelial differentiation-related factor 1; endothelial differentiation related factor 1; anti-EDF-1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-EDF-1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 98 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human EDF-1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-EDF-1 antibody AAA212467 (lot AAA212467-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human EDF-1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-EDF-1 antibody AAA212467 (lot AAA212467-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human EDF-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-EDF-1 antibody AAA212467 (lot AAA212467-1) used for IP at 6 ug/mg lysate. EDF-1 was also immunoprecipitated by rabbit anti-EDF-1 antibody BL14527. For blotting immunoprecipitated EDF-1, AAA212467 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human EDF-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-EDF-1 antibody AAA212467 (lot AAA212467-1) used for IP at 6 ug/mg lysate. EDF-1 was also immunoprecipitated by rabbit anti-EDF-1 antibody BL14527. For blotting immunoprecipitated EDF-1, AAA212467 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human EDF-1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EDF-1 (Cat. No. AAA212467 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human EDF-1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EDF-1 (Cat. No. AAA212467 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-EDF-1 antibody
Endothelial differentiation-related factor 1 (EDF-1) is a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. EDF-1 has also been found to act as a transcriptional co-activator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators [taken from NCBI Entrez Gene (Gene ID: 8721)].
Product Categories/Family for anti-EDF-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17.4 kDa (156aa) confirmed by MALDI-TOF
NCBI Official Full Name
endothelial differentiation-related factor 1 isoform beta
NCBI Official Synonym Full Names
endothelial differentiation-related factor 1
NCBI Official Symbol
EDF1
NCBI Official Synonym Symbols
MBF1; EDF-1
NCBI Protein Information
endothelial differentiation-related factor 1
UniProt Protein Name
Endothelial differentiation-related factor 1
UniProt Gene Name
EDF1
UniProt Synonym Gene Names
EDF-1; MBF1
UniProt Entry Name
EDF1_HUMAN
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Product Notes
The EDF-1 edf1 (Catalog #AAA212467) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-EDF-1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EDF-1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the EDF-1 edf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EDF-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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