FCM/FACS (Flow Cytometry)
(EFR3A Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human EFR3A Polyclonal Antibody | anti-EFR3A antibody
EFR3A Antibody (C-term)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
EFR3A, Antibody; EFR3A Antibody (C-term); Protein EFR3 homolog A; Protein EFR3-like; EFR3A; KIAA0143; anti-EFR3A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This EFR3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636-664 amino acids from the C-terminal region of human EFR3A.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
636-664
Sequence Length
821
Applicable Applications for anti-EFR3A antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Function
Component of a complex required to localize phosphatidylinositol 4-kinase (PI4K) to the plasma membrane (PubMed:23229899, PubMed:25608530, PubMed:26571211). The complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis (Probable). In the complex, EFR3A probably acts as the membrane-anchoring component (PubMed:23229899). Also involved in responsiveness to G-protein-coupled receptors; it is however unclear whether this role is direct or indirect (PubMed:25380825).
Cellular Location
Cell membrane; Lipid-anchor. Cytoplasm, cytosol. Note=Palmitoylation anchors the protein to the plasma membrane (PubMed:23229899, PubMed:25380825, PubMed:26571211). A small amount is observed in the cytosol (PubMed:25380825).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(EFR3A Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(EFR3A Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of EFR3A Antibody (C-term) in K562 cell line lysates (35ug/lane). EFR3A (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of EFR3A Antibody (C-term) in K562 cell line lysates (35ug/lane). EFR3A (arrow) was detected using the purified Pab.)
Product Categories/Family for anti-EFR3A antibody
References
Munemoto, Y., et al. Brain Res. Mol. Brain Res. 128(2):131-140(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein EFR3 homolog A
NCBI Official Synonym Full Names
EFR3 homolog A (S. cerevisiae)
NCBI Official Symbol
EFR3A
NCBI Protein Information
protein EFR3 homolog A
UniProt Protein Name
Protein EFR3 homolog A
UniProt Gene Name
EFR3A
UniProt Synonym Gene Names
KIAA0143
UniProt Entry Name
EFR3A_HUMAN
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Product Notes
The EFR3A efr3a (Catalog #AAA287967) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 636-664. The EFR3A Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EFR3A can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the EFR3A efr3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EFR3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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