FCM/FACS (Flow Cytometry)
(EIF2A Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human EIF2A Polyclonal Antibody | anti-EIF2A antibody
EIF2A Antibody (Center)
Gene Names
EIF2A; CDA02; EIF-2A; MST089; MSTP004; MSTP089
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EIF2A, Antibody; EIF2A Antibody (Center); Eukaryotic translation initiation factor 2A; eIF-2A; 65 kDa eukaryotic translation initiation factor 2A; N-terminally processed; EIF2A; anti-EIF2A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EIF2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-465 amino acids from the Central region of human EIF2A.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Positions
437-465
Sequence Length
585
Applicable Applications for anti-EIF2A antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(EIF2A Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(EIF2A Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with EIF2A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with EIF2A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of EIF2A Antibody (Center) in Ramos cell line lysates (35ug/lane). EIF2A (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of EIF2A Antibody (Center) in Ramos cell line lysates (35ug/lane). EIF2A (arrow) was detected using the purified Pab.)
Related Product Information for anti-EIF2A antibody
EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).
References
Willis, K.L., et al. Virology 394(1):73-81(2009)
Spurgeon, M.E., et al. J. Virol. 83(19):9970-9982(2009)
Lu, W., et al. J. Biol. Chem. 284(36):24281-24288(2009)
Yang, J., et al. Mol. Cell. Biol. 29(8):2243-2253(2009)
Schewe, D.M., et al. Cancer Res. 69(4):1545-1552(2009)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Spurgeon, M.E., et al. J. Virol. 83(19):9970-9982(2009)
Lu, W., et al. J. Biol. Chem. 284(36):24281-24288(2009)
Yang, J., et al. Mol. Cell. Biol. 29(8):2243-2253(2009)
Schewe, D.M., et al. Cancer Res. 69(4):1545-1552(2009)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64990
NCBI Official Full Name
eukaryotic translation initiation factor 2A
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2A, 65kDa
NCBI Official Symbol
EIF2A
NCBI Official Synonym Symbols
CDA02; EIF-2A; MST089; MSTP004; MSTP089
NCBI Protein Information
eukaryotic translation initiation factor 2A
UniProt Protein Name
Eukaryotic translation initiation factor 2A
UniProt Gene Name
EIF2A
UniProt Synonym Gene Names
eIF-2A
UniProt Entry Name
EIF2A_HUMAN
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Product Notes
The EIF2A eif2a (Catalog #AAA285903) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 437-465. The EIF2A Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF2A can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the EIF2A eif2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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