IP (Immunoprecipitation)
(Immunoprecipitating EIF3F in Jurkat whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA231997 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)Lane 2: AAA231997(4ug)+ Jurkat whole cell lysate(500ug)Lane 3: Jurkat whole cell lysate (20ug))
Rabbit anti-Human EIF3F Polyclonal Antibody | anti-EIF3F antibody
EIF3F Antibody
Gene Names
EIF3F; EIF3S5; eIF3-p47
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA
Purity
Antigen Affinity Purified
Synonyms
EIF3F, Antibody; EIF3F Antibody; Eukaryotic translation initiation factor 3 subunit F; Deubiquitinating enzyme eIF3f; Eukaryotic translation initiation factor 3 subunit 5; EIF3F; anti-EIF3F antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
357
Applicable Applications for anti-EIF3F antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA
Species
Human
Immunogen
Recombinant human Eukaryotic translation initiation factor 3 subunit F protein (C-270AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-30248 / sc-30247
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating EIF3F in Jurkat whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA231997 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)Lane 2: AAA231997(4ug)+ Jurkat whole cell lysate(500ug)Lane 3: Jurkat whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating EIF3F in Jurkat whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA231997 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)Lane 2: AAA231997(4ug)+ Jurkat whole cell lysate(500ug)Lane 3: Jurkat whole cell lysate (20ug))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using AAA231997 at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using AAA231997 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human brain tissue using AAA231997 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human brain tissue using AAA231997 at dilution 1:100)
Related Product Information for anti-EIF3F antibody
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,564 Da
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit F
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3 subunit F
NCBI Official Symbol
EIF3F
NCBI Official Synonym Symbols
EIF3S5; eIF3-p47
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit F
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit F
UniProt Gene Name
EIF3F
UniProt Synonym Gene Names
eIF3f
UniProt Entry Name
EIF3F_HUMAN
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EIF3F Antibody
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Reactivity: Human, Mouse
Apps: Immunohistochemistry, Western Blot, ELISA
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Host: Rabbit
Reactivity: Human, Mouse
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Product Notes
The EIF3F eif3f (Catalog #AAA231997) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF3F Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF3F can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the EIF3F eif3f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF3F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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