WB (Western Blot)
(Detection of human EMSY by western blot. Samples: Whole cell lysate (50 ug) from BeWo, AlphaTC1 Clone 9, A20, 3T3-L1, and cells. Antibody: Affinity purified rabbit anti-EMSY antibody EMSY (AAA210844 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human EMSY Polyclonal Antibody | anti-EMSY antibody
Rabbit anti-EMSY Antibody, Affinity Purified
Gene Names
C11orf30; EMSY; GL002
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
EMSY, Antibody; Rabbit anti-EMSY Antibody, Affinity Purified; BRCA2-interacting transcriptional repressor EMSY; C11orf30; GL002; protein EMSY; EMSY, BRCA2 interacting transcriptional repressor; anti-EMSY antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-EMSY antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1272 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human EMSY by western blot. Samples: Whole cell lysate (50 ug) from BeWo, AlphaTC1 Clone 9, A20, 3T3-L1, and cells. Antibody: Affinity purified rabbit anti-EMSY antibody EMSY (AAA210844 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human EMSY by western blot. Samples: Whole cell lysate (50 ug) from BeWo, AlphaTC1 Clone 9, A20, 3T3-L1, and cells. Antibody: Affinity purified rabbit anti-EMSY antibody EMSY (AAA210844 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human EMSY by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EMSY antibody (AAA210844 lot 3) used for IP at 6 ug per reaction. EMSY was also immunoprecipitated by a previous lot of this antibody (AAA210844 lot 2) and another antibody against a different epitope of EMSY (BL1049). For blotting immunoprecipitated EMSY, AAA210844 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human EMSY by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EMSY antibody (AAA210844 lot 3) used for IP at 6 ug per reaction. EMSY was also immunoprecipitated by a previous lot of this antibody (AAA210844 lot 2) and another antibody against a different epitope of EMSY (BL1049). For blotting immunoprecipitated EMSY, AAA210844 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human EMSY by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EMSY (Cat. No. AAA210844 Lot3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human EMSY by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EMSY (Cat. No. AAA210844 Lot3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-EMSY antibody
EMSY has been identified as a protein that interacts with BRCA2 and suppresses BRCA2 transactivational activity. EMSY is amplified in some cases of sporadic human breast and ovarian cancer indicating a role in tumorigenesis that is linked to BRCA2 function. EMSY can also associate with HP1beta and BS69 and may be involved in chromatin remodeling.
Product Categories/Family for anti-EMSY antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
141,468 Da
NCBI Official Full Name
protein EMSY isoform 4
NCBI Official Synonym Full Names
chromosome 11 open reading frame 30
NCBI Official Symbol
C11orf30
NCBI Official Synonym Symbols
EMSY; GL002
NCBI Protein Information
protein EMSY
UniProt Protein Name
Protein EMSY
UniProt Gene Name
EMSY
UniProt Synonym Gene Names
C11orf30
UniProt Entry Name
EMSY_HUMAN
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Product Notes
The EMSY emsy (Catalog #AAA210844) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-EMSY Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EMSY can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the EMSY emsy for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EMSY, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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