IHC (Immunohistochemisry)
(ENO2/NSE Antibody-Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit ENO2/NSE Polyclonal Antibody | anti-ENO2 antibody
ENO2/NSE Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Gene Names
ENO2; NSE
Reactivity
Chicken, Zebrafish, Mouse, Rat, Human, Monkey
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Immunoaffinity purified
Synonyms
ENO2/NSE, Antibody; ENO2/NSE Rabbit anti-Human Polyclonal (C-Terminus) Antibody; PathPlus ENO2/NSE Antibody (C-Terminus); ENO2; Enolase 2; Enolase 2 (gamma; neuronal); Neurone-specific enolase; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE; anti-ENO2 antibody
Host
Rabbit
Reactivity
Chicken, Zebrafish, Mouse, Rat, Human, Monkey
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Gamma-enolase protein.
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, pH7.3, 0.01% Sodium Azide, 30% Glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ENO2 antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry)
Target
Human ENO2/NSE
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human Gamma-enolase.
Conjugation
Unconjugated
Epitope
C-Terminus
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
IHC (Immunohistochemisry)
(ENO2/NSE Antibody-Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemisry)
(ENO2/NSE Antibody-Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(ENO2/NSE Antibody-Western blot analysis of Gamma-enolase expression in U937 (A); K562 (B); Jurkat (C); NIH3T3 (D); KNRK (E); HepG2 (F); HeLa (G) whole cell lysates.)
WB (Western Blot)
(ENO2/NSE Antibody-Western blot analysis of Gamma-enolase expression in U937 (A); K562 (B); Jurkat (C); NIH3T3 (D); KNRK (E); HepG2 (F); HeLa (G) whole cell lysates.)
ICC (Immunocytochemistry)
(ENO2/NSE Antibody-Immunofluorescent analysis of Gamma-enolase staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
ICC (Immunocytochemistry)
(ENO2/NSE Antibody-Immunofluorescent analysis of Gamma-enolase staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Related Product Information for anti-ENO2 antibody
NSE antibody is an unconjugated rabbit polyclonal antibody to NSE (ENO2)(C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, ICC, IF, IHC and WB.
NSE (neuron-specific enolase, ENO2) is a metalloenzyme involved in neural tissue development. In immunohistochemistry, NSE is a positive marker for neurons and neuroendocrine cells, and it is also positive in islets of Langerhans of the pancreas. In cancers, it is positive in neuroendocrine tumors such as carcinoid and schwannoma. NSE is a useful marker of disease status and prognosis in small-cell lung cancer, prostate cancer and acute lymphoblastic leukemia where elevated levels may indicate progression.
NSE (neuron-specific enolase, ENO2) is a metalloenzyme involved in neural tissue development. In immunohistochemistry, NSE is a positive marker for neurons and neuroendocrine cells, and it is also positive in islets of Langerhans of the pancreas. In cancers, it is positive in neuroendocrine tumors such as carcinoid and schwannoma. NSE is a useful marker of disease status and prognosis in small-cell lung cancer, prostate cancer and acute lymphoblastic leukemia where elevated levels may indicate progression.
References
Br J Cancer. 1994 Oct; 70(4): 759 -761, PMID: 7917935; Int J Biol Markers. 2018 Jan;33(1):10-21, PMID: 28885659; Cell Physiol Biochem. 2018;46(4):1525-1535, PMID: 29689546; Int J Clin Exp Med. 2015; 8(6): 9498 -9504, PMID: 26309614
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,269 Da
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2 (gamma, neuronal)
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE
NCBI Protein Information
gamma-enolase; neural enolase; neuron-specific enolase; neurone-specific enolase; neuron specific gamma enolase; 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase
UniProt Protein Name
Gamma-enolase
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
UniProt Entry Name
ENOG_HUMAN
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Product Notes
The ENO2 eno2 (Catalog #AAA162683) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENO2/NSE Rabbit anti-Human Polyclonal (C-Terminus) Antibody reacts with Chicken, Zebrafish, Mouse, Rat, Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's ENO2/NSE can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the ENO2 eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENO2/NSE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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