IF (Immunofluorescence)
(Immunofluorescent analysis of eNOS (pS1177) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Rabbit eNOS (pS1177) Polyclonal Antibody | anti-NOS3 antibody
Anti-eNOS (pS1177) Phospho Antibody
Gene Names
NOS3; eNOS; ECNOS
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep
Applications
Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
eNOS (pS1177), Antibody; Anti-eNOS (pS1177) Phospho Antibody; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII; anti-NOS3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of eNOS (pS1177) protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
1203
Applicable Applications for anti-NOS3 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human eNOS. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of eNOS (pS1177) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of eNOS (pS1177) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
WB (Western Blot)
(Western blot analysis of eNOS (pS1177) expression in A549 (A), HuvEc (B), rat brain (C) whole cell lysates.)
WB (Western Blot)
(Western blot analysis of eNOS (pS1177) expression in A549 (A), HuvEc (B), rat brain (C) whole cell lysates.)
Related Product Information for anti-NOS3 antibody
Rabbit polyclonal antibody to eNOS (pS1177)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nitric oxide synthase, endothelial isoform 1
NCBI Official Synonym Full Names
nitric oxide synthase 3 (endothelial cell)
NCBI Official Symbol
NOS3
NCBI Official Synonym Symbols
eNOS; ECNOS
NCBI Protein Information
nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3 transcript variant eNOS-delta20-21
UniProt Protein Name
Nitric oxide synthase, endothelial
UniProt Gene Name
NOS3
UniProt Synonym Gene Names
cNOS; eNOS; NOSIII
UniProt Entry Name
NOS3_HUMAN
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Product Notes
The NOS3 nos3 (Catalog #AAA104941) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-eNOS (pS1177) Phospho Antibody reacts with Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's eNOS (pS1177) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the NOS3 nos3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eNOS (pS1177), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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