Rabbit NOS3 / eNOS Polyclonal Antibody | anti-NOS3 antibody

Rabbit Polyclonal (IgG) to Human NOS3 / eNOS

Cat. Num. AAA52139

• Gene Names: NOS3; eNOS; ECNOS
• Reactivity: Gorilla, Human, Monkey, Rabbit; Predicted Reactivity: Mouse, Rat, Bovine, Guinea pig, Hamster (at least 90% immunogen sequence identity)
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A Purified
• Synonyms: NOS3 / eNOS, Antibody; Rabbit Polyclonal (IgG) to Human NOS3 / eNOS; Anti-NOS3 / eNOS Antibody (aa1181-1194) IHC-plus; NOS3; Constitutive NOS; CNOS; Endothelial NOS; ECNOS; NOS type III; NOSIII; EC-NOS; ENOS; Nitric oxide synthase 3; Human NOS3; eNOS; anti-NOS3 antibody

• Host: Rabbit
• Reactivity: Gorilla, Human, Monkey, Rabbit; Predicted Reactivity: Mouse, Rat, Bovine, Guinea pig, Hamster (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes eNOS/NOS III, Mr ~135kD. Species cross-reactivity: Human and bovine. Predicted to cross-react with mouse, guinea pig, porcine, rat and canine based on sequence homology.
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.6, 0.07% sodium azide, before the addition of glycerol to 30%
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1203

• Applicable Applications for anti-NOS3 antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Target Species: Human
• Immunogen Description: KLH-conjugated, synthetic peptide corresponding to aa1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, porcine, rat and canine.
• Immunogen Type: Synthetic peptide - KLH conjugated
• Immunogen: NOS3 / eNOS antibody was raised against kLH-conjugated, synthetic peptide corresponding to aa1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, porcine, rat and canine. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Elephant, Rabbit (100%); Mouse, Rat, Hamster, Panda, Bovine, Guinea pig (93%); Dog, Bat, Pig (86%).
• Antigen Modification: aa1181-1194
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C

IHC (Immunohiostchemistry)

(Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry)

(Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-NOS3 antibody

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 40254422
• NCBI GeneID: 4846
• NCBI Accession #: NP_000594.2
• NCBI GenBank Nucleotide #: NM_000603.4
• UniProt Accession #: P29474; Q13662; Q14251; Q14434; Q548C1; Q6GSL5; Q9UDC6; A0S0A7; A0S0A8; A8KA63; B2RCQ1; E9PFR2
• Molecular Weight: 67,876 Da
• NCBI Official Full Name: nitric oxide synthase, endothelial isoform 1
• NCBI Official Synonym Full Names: nitric oxide synthase 3 (endothelial cell)
• NCBI Official Symbol: NOS3
• NCBI Official Synonym Symbols: eNOS; ECNOS
• NCBI Protein Information: nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3 transcript variant eNOS-delta20-21
• UniProt Protein Name: Nitric oxide synthase, endothelial
• UniProt Gene Name: NOS3
• UniProt Synonym Gene Names: cNOS; eNOS; NOSIII
• UniProt Entry Name: NOS3_HUMAN