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FCM (Flow Cytometry)
(ENPP2 Antibody (Center)(AAA28662) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human ENPP2 Polyclonal Antibody | anti-ENPP2 antibody
ENPP2 Antibody (Center)
Gene Names
ENPP2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
ENPP2, Antibody; ENPP2 Antibody (Center); Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; Autotaxin; Extracellular lysophospholipase D; LysoPLD; ENPP2; ATX; PDNP2; anti-ENPP2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This ENPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the Central region of human ENPP2.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
888
Applicable Applications for anti-ENPP2 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC)
Application Notes
WB~~1:2000
FC~~1:10~50
FC~~1:10~50
Antigen Region
377-406
Tissue Location
Detected in blood plasma (at protein level)(PubMed:12176993, PubMed:26371182). Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.
Function
Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931, PubMed:14500380, PubMed:12354767,). Major substrate is lysophosphatidylcholine (PubMed:12176993, PubMed:27754931, PubMed:14500380). Also can act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility (PubMed:14500380). Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed:15769751, PubMed:12176993). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed:11559573). Stimulates migration of melanoma cells,probably via a pertussis toxin-sensitive G
protein (PubMed:1733949). May have a role in induction of parturition (PubMed:12176993). Possible involvement in cell proliferation and adipose tissue development (Probable). Tumor cell motility-stimulating factor (PubMed:1733949, PubMed:11559573). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids (PubMed:21393252). Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3- phosphocholine (lyso-PAF) (PubMed:21393252).
Cellular Location
Secreted
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM (Flow Cytometry)
(ENPP2 Antibody (Center)(AAA28662) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM (Flow Cytometry)
(ENPP2 Antibody (Center)(AAA28662) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of lysates from human placenta and plasma tissue lysate (from left to right), using ENPP2 Antibody (Center)(Cat. #AAA28662). AAA28662 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from human placenta and plasma tissue lysate (from left to right), using ENPP2 Antibody (Center)(Cat. #AAA28662). AAA28662 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of (arrow) using rabbit polyclonal ENPP2 Antibody (Center)(AAA28662). 293T cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the gene.)
WB (Western Blot)
(Western blot analysis of (arrow) using rabbit polyclonal ENPP2 Antibody (Center)(AAA28662). 293T cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the gene.)
WB (Western Blot)
(Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 99 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 99 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 99 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 99 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-ENPP2 antibody
ENPP2 functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This protein stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The protein is secreted and further processed to make the biologically active form.
Product Categories/Family for anti-ENPP2 antibody
References
Kawagoe H., Soma O., Goji J., Nishimura N., Narita M.,Genomics 30:380-384(1995)
Nam S.W., Clair T., Kim Y.S., McMarlin A.,Cancer Res. 61:6938-6944(2001)
The MGC Project Team Genome Res. 14:2121-2127(2004)
Nam S.W., Clair T., Kim Y.S., McMarlin A.,Cancer Res. 61:6938-6944(2001)
The MGC Project Team Genome Res. 14:2121-2127(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform 2 preproprotein
NCBI Official Synonym Full Names
ectonucleotide pyrophosphatase/phosphodiesterase 2
NCBI Official Symbol
ENPP2
NCBI Official Synonym Symbols
ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
NCBI Protein Information
ectonucleotide pyrophosphatase/phosphodiesterase family member 2
UniProt Protein Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
UniProt Gene Name
ENPP2
UniProt Synonym Gene Names
ATX; PDNP2; E-NPP 2; LysoPLD
UniProt Entry Name
ENPP2_HUMAN
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Product Notes
The ENPP2 enpp2 (Catalog #AAA28662) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENPP2 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENPP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC). WB~~1:2000 FC~~1:10~50. Researchers should empirically determine the suitability of the ENPP2 enpp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENPP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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