IP (Immunoprecipitation)
(ENSA was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate2 uL anti-ENSA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ENSA rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 13 kDaObserved band size: 15 kDa)
Rabbit anti-Human ENSA Polyclonal Antibody | anti-ENSA antibody
Anti-ENSA Antibody, Rabbit Polyclonal
Gene Names
ENSA; ARPP-19e
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
ENSA, Antibody; Anti-ENSA Antibody, Rabbit Polyclonal; ENSA/Endosulfine alpha Antibody, Rabbit PAb, Antigen Affinity Purified; endosulfine alpha; Anti-ARPP-19e Antibody; anti-ENSA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ENSA/Endosulfine alpha
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ENSA antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human ENSA/Endosulfine alpha protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human ENSA/Endosulfine alpha (rh ENSA/Endosulfine alpha). ENSA/Endosulfine alpha specific IgG was purified by Human ENSA/Endosulfine alpha affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(ENSA was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate2 uL anti-ENSA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ENSA rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 13 kDaObserved band size: 15 kDa)
IP (Immunoprecipitation)
(ENSA was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate2 uL anti-ENSA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ENSA rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 13 kDaObserved band size: 15 kDa)
WB (Western Blot)
(Anti-ENSA rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse heart tissue lysate Whole Cell LysateLane B: Rat heart tissue lysate Whole Cell LysateLane C: Mouse brain tissue lysate Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:26 kDa)
WB (Western Blot)
(Anti-ENSA rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse heart tissue lysate Whole Cell LysateLane B: Rat heart tissue lysate Whole Cell LysateLane C: Mouse brain tissue lysate Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:26 kDa)
Related Product Information for anti-ENSA antibody
Endosulfine alpha, also known as ENSA, belongs to the endosulfine family. It is a highly conserved cAMP-regulated phosphoprotein (ARPP) family. Endosulfine alpha is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. As a protein phosphatase inhibitor, ENSA specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase By similarity. Endosulfine alpha also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.
References
Ye M. et al., 2001, Genome Res. 10 (10): 1546-60. Apiou F. et al., 1999, Diabetes 48 (9): 1873-6. Lennon G. et al., 1997, Genome Res. 6 (9): 791-806.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,868 Da
NCBI Official Full Name
alpha-endosulfine isoform 3
NCBI Official Synonym Full Names
endosulfine alpha
NCBI Official Symbol
ENSA
NCBI Official Synonym Symbols
ARPP-19e
NCBI Protein Information
alpha-endosulfine
UniProt Protein Name
Alpha-endosulfine
UniProt Gene Name
ENSA
UniProt Entry Name
ENSA_HUMAN
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Product Notes
The ENSA ensa (Catalog #AAA255754) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ENSA Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENSA can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ENSA ensa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENSA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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