FCM/FACS (Flow Cytometry)
(ENSA Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ENSA Polyclonal Antibody | anti-ENSA antibody
ENSA Antibody (Center)
Gene Names
ENSA; ARPP-19e
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken, Zebrafish, Pig, Rat, Xenopus)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
ENSA, Antibody; ENSA Antibody (Center); Alpha-endosulfine; ARPP-19e; ENSA; anti-ENSA antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken, Zebrafish, Pig, Rat, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ENSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the Central region of human ENSA.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
39-66
Sequence Length
133
Applicable Applications for anti-ENSA antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Function
Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.
Cellular Location
Cytoplasm.
Tissue Location
Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ENSA Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ENSA Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(ENSA Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the ENSA antibody detected the ENSA protein (arrow).)
WB (Western Blot)
(ENSA Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the ENSA antibody detected the ENSA protein (arrow).)
WB (Western Blot)
(ENSA Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ENSA antibody detected the ENSA protein (arrow).)
WB (Western Blot)
(ENSA Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ENSA antibody detected the ENSA protein (arrow).)
Related Product Information for anti-ENSA antibody
ENSA belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes.
Product Categories/Family for anti-ENSA antibody
References
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Gabrielsson, B.G., et al. Mol. Cell. Biochem. 258 (1-2), 65-71 (2004) :
Thameem, F., et al. Mol. Genet. Metab. 81(1):16-21(2004)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Gabrielsson, B.G., et al. Mol. Cell. Biochem. 258 (1-2), 65-71 (2004) :
Thameem, F., et al. Mol. Genet. Metab. 81(1):16-21(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13389
NCBI Official Full Name
alpha-endosulfine isoform 3
NCBI Official Synonym Full Names
endosulfine alpha
NCBI Official Symbol
ENSA
NCBI Official Synonym Symbols
ARPP-19e
NCBI Protein Information
alpha-endosulfine
UniProt Protein Name
Alpha-endosulfine
UniProt Gene Name
ENSA
UniProt Entry Name
ENSA_HUMAN
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Product Notes
The ENSA ensa (Catalog #AAA289386) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 39-66. The ENSA Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Bovine, Chicken, Zebrafish, Pig, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's ENSA can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ENSA ensa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENSA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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