Rabbit EPAC1 Polyclonal Antibody | anti-RAPGEF3 antibody

EPAC1 Antibody

Cat. Num. AAA39301

• Gene Names: RAPGEF3; EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: EPAC1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: EPAC1, Antibody; EPAC1 Antibody; EPAC1; EPAC; bcm910; HSU79275; CAMP-GEFI; CGEF1; Rap guanine nucleotide exchange factor 3; Exchange factor directly activated by cAMP 1; EPAC 1; Rap guanine nucleotide exchange factor (GEF) 3; anti-RAPGEF3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two isoforms of EPAC1 are known to exist; this antibody will detect both isoforms. EPAC1 antibody is predicted to not cross-react with EPAC2.
• Purity/Purification: EPAC1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 923

• Applicable Applications for anti-RAPGEF3 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: EPAC1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EPAC1.
• Buffer: EPAC1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: EPAC1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of EPAC1 in rat brain tissue with EPAC1 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of EPAC1 in rat brain tissue with EPAC1 antibody at 2.5 μg/mL.)

WB (Western Blot)

(Western blot analysis of EPAC1 in rat skeletal muscle tissue lysate with EPAC1 antibody at 1 μg/mL.)

Related Product Information for anti-RAPGEF3 antibody

EPAC1 Antibody: EPAC1, also known as Rap guanine nuclear exchange factor 3 and cAMPGEF-I, is widely expressed but most prominently in brain, heart, kidney, pancreas, spleen, ovary, thyroid and spinal cord. EPAC1 is a cAMP-binding protein with intrinsic guanine nuclear exchange factor activity that couples cAMP production to the activation of Rap, a GTPase belonging to the Ras family. This activation of Rap influences numerous cellular processes such as integrin-mediated cell adhesion, vascular endothelial barrier formation, and cardiac myocyte gap junction formation. Recently, EPAC1 has been suggested to also be involved in the cAMP-dependent regulation of ion channel formation, intracellular Ca++ signalling, ion transporter activity, and exocytosis.

NCBI and Uniprot Product Information

• NCBI GI #: 148747859
• NCBI GeneID: 10411
• NCBI Accession #: NP_659099
• NCBI GenBank Nucleotide #: NM_001098531.2
• UniProt Accession #: O95398; O95634; Q8WVN0; A8K2G5; E7EQC8
• Molecular Weight: 99,452 Da
• NCBI Official Full Name: rap guanine nucleotide exchange factor 3 isoform a
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor (GEF) 3
• NCBI Official Symbol: RAPGEF3
• NCBI Official Synonym Symbols: EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI
• NCBI Protein Information: rap guanine nucleotide exchange factor 3; EPAC 1; 9330170P05Rik; exchange factor directly activated by cAMP 1; exchange protein directly activated by cAMP 1; cAMP-regulated guanine nucleotide exchange factor I; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP
• UniProt Protein Name: Rap guanine nucleotide exchange factor 3
• UniProt Gene Name: RAPGEF3
• UniProt Synonym Gene Names: CGEF1; EPAC; EPAC1; EPAC 1; cAMP-GEFI
• UniProt Entry Name: RPGF3_HUMAN