IF (Immunofluorescence)
(Immunofluorescent analysis of Ephrin B3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Rabbit Ephrin B3 Polyclonal Antibody | anti-EFNB3 antibody
Anti-Ephrin B3 Antibody
Gene Names
EFNB3; EFL6; EPLG8; LERK8
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
Ephrin B3, Antibody; Anti-Ephrin B3 Antibody; EPLG8; LERK8; Ephrin-B3; EPH-related receptor transmembrane ligand ELK-L3; EPH-related receptor tyrosine kinase ligand 8; LERK-8; anti-EFNB3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Ephrin B3 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
340
Applicable Applications for anti-EFNB3 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Ephrin B3. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of Ephrin B3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of Ephrin B3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IHC (Immunohiostchemistry)
IHC (Immunohiostchemistry)
WB (Western Blot)
(Western blot analysis of Ephrin B3 expression in HepG2 (A), NIH3T3 (B), mouse liver (C), rat liver (D) whole cell lysates.)
WB (Western Blot)
(Western blot analysis of Ephrin B3 expression in HepG2 (A), NIH3T3 (B), mouse liver (C), rat liver (D) whole cell lysates.)
Related Product Information for anti-EFNB3 antibody
Rabbit polyclonal antibody to Ephrin B3
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,835 Da
NCBI Official Full Name
ephrin-B3
NCBI Official Synonym Full Names
ephrin-B3
NCBI Official Symbol
EFNB3
NCBI Official Synonym Symbols
EFL6; EPLG8; LERK8
NCBI Protein Information
ephrin-B3; Ephrin B3; eph-related receptor tyrosine kinase ligand 8; EPH-related receptor transmembrane ligand ELK-L3
UniProt Protein Name
Ephrin-B3
UniProt Gene Name
EFNB3
UniProt Synonym Gene Names
EPLG8; LERK8; LERK-8
UniProt Entry Name
EFNB3_HUMAN
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Product Notes
The EFNB3 efnb3 (Catalog #AAA105075) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ephrin B3 Antibody reacts with Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Ephrin B3 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the EFNB3 efnb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ephrin B3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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