Rabbit anti-Human ER alpha Polyclonal Antibody | anti-ESR1 antibody

Anti-ER alpha Antibody, Rabbit Polyclonal

Cat. Num. AAA254213

• Gene Names: ESR1; ER; ESR; Era; ESRA; NR3A1
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: ER alpha, Antibody; Anti-ER alpha Antibody, Rabbit Polyclonal; ER alpha/ESR1 Antibody, Rabbit PAb, Antigen Affinity Purified; estrogen receptor 1; Anti-ER Antibody; Anti-Era Antibody; Anti-ESR Antibody; Anti-ESRA Antibody; Anti-ESTRR Antibody; Anti-NR3A1 Antibody; anti-ESR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ER alpha/ESR1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ESR1 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
• Immunogen: A synthetic peptide corresponding to the center region of the Human ER alpha/ESR1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ER alpha/ESR1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IHC (Immunohistochemisry)

(Immunochemical staining of human ER alpha/ESR1 in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

IHC (Immunohiostchemistry)

(Immunochemical staining of human ER alpha/ESR1 in human endometrial cancer (from 2 donors) with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

IF (Immunofluorescence)

(Immunofluorescence staining of ER alpha/ESR1 in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ER alpha/ESR1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)

Related Product Information for anti-ESR1 antibody

An ESR1 gene mutation, which encodes for the estrogen receptor (ER) protein, is one of the potential mechanisms of therapy resistance. The ESR1 mutations result in conformational changes in the ER leading to subsequent estrogen-independent transcriptional activity. The mutations within the estrogen receptor (ER) gene, ESR1, frequently occur in metastatic breast cancer and influence response to hormone therapy. Estrogen receptor alpha (ERalpha) is one of the estrogen hormone-activated transcription factors, which regulates a large number of genes and is involved in the mammary gland development. Expression of ERalpha is considered to be a good indicator for endocrine therapy and breast cancer survival. Loss of ERalpha in breast cancer patients indicates invasiveness and poor prognosis. ESR1 as a direct target of miR-301a-3p and suggest that miR-301a-3p likely contributes to development of estrogen independence, which leads to a more invasive phenotype of breast cancer. Polymorphisms of the estrogen receptor gene alpha (ESR1) are consistently been associated with bone mineral density (BMD) and fracture.

Product Categories/Family for anti-ESR1 antibody

MAPK-Erk Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 62821794
• NCBI GeneID: 2099
• NCBI Accession #: NP_000116.2
• NCBI GenBank Nucleotide #: NM_000125.3
• UniProt Accession #: P03372; Q13511; Q14276; Q5T5H7; Q6MZQ9; Q9NU51; Q9UDZ7; Q9UIS7
• Molecular Weight: 66,216 Da
• NCBI Official Full Name: estrogen receptor
• NCBI Official Synonym Full Names: estrogen receptor 1
• NCBI Official Symbol: ESR1
• NCBI Official Synonym Symbols: ER; ESR; Era; ESRA; NR3A1
• NCBI Protein Information: estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha delta 4 +49 isoform; nuclear receptor subfamily 3 group A member 1; estrogen
• UniProt Protein Name: Estrogen receptor
• UniProt Gene Name: ESR1
• UniProt Synonym Gene Names: ESR; NR3A1; ER
• UniProt Entry Name: ESR1_HUMAN