WB (Western Blot)
(Detection of human ERCC5/XPG by western blot. Samples: Whole cell lysate (50 ug) from RKO, A-549, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC5/XPG antibody (AAA211499 lot 3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human ERCC5/XPG Polyclonal Antibody | anti-ERCC5/XPG antibody
Rabbit anti-ERCC5/XPG Antibody, Affinity Purified
Gene Names
ERCC5; XPG; UVDR; XPGC; COFS3; ERCM2
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ERCC5/XPG, Antibody; Rabbit anti-ERCC5/XPG Antibody, Affinity Purified; excision repair cross-complementing rodent repair deficiency, complementation group 5; XPGC; XPG; xeroderma pigmentosum, complementation group G; Xeroderma pigmentosum group G-complementing protein; UVDR; ERCM2; ERCC5-201; DNA repair protein complementing XP-G cells; DNA excision repair protein ERCC-5; COFS3; excision repair cross-complementation group 5; XPG-complementing protein; ERCC excision repair 5, endonuclease; anti-ERCC5/XPG antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ERCC5/XPG antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 650 and 700
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ERCC5/XPG by western blot. Samples: Whole cell lysate (50 ug) from RKO, A-549, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC5/XPG antibody (AAA211499 lot 3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human ERCC5/XPG by western blot. Samples: Whole cell lysate (50 ug) from RKO, A-549, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC5/XPG antibody (AAA211499 lot 3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human ERCC5/XPG by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERCC5/XPG antibody (AAA211499 lot 3) used for IP at 6 ug per reaction. ERCC5/XPG was also immunoprecipitated by a previous lot of this antibody (AAA211499 lot 2) and a second antibody against a different epitope of ERCC5/XPG . For blotting immunoprecipitated ERCC5/XPG, AAA211499 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human ERCC5/XPG by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERCC5/XPG antibody (AAA211499 lot 3) used for IP at 6 ug per reaction. ERCC5/XPG was also immunoprecipitated by a previous lot of this antibody (AAA211499 lot 2) and a second antibody against a different epitope of ERCC5/XPG . For blotting immunoprecipitated ERCC5/XPG, AAA211499 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human ERCC5/XPG by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ERCC5/XPG (AAA211499 lot 3) used at a dilution of 1:1000 (1 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human ERCC5/XPG by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ERCC5/XPG (AAA211499 lot 3) used at a dilution of 1:1000 (1 ug/ml). Detection: DAB)
Related Product Information for anti-ERCC5/XPG antibody
ERCC5 is a single-stranded DNA endonuclease involved in DNA excision repair. ERCC5 is an important component of the nucleotide excision repair (NER) pathway which is critical to the repair of DNA lesions caused by exposure to UV light. Defects in ERCC5 are the cause of xeroderma pigmentosum, an autosomal recessive disease characterized by a hypersensitivity to sunlight and a high predisposition to cancer on UV-exposed areas.
Product Categories/Family for anti-ERCC5/XPG antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
133,108 Da
NCBI Official Full Name
DNA repair protein complementing XP-G cells
NCBI Official Synonym Full Names
excision repair cross-complementing rodent repair deficiency, complementation group 5
NCBI Official Symbol
ERCC5
NCBI Official Synonym Symbols
XPG; UVDR; XPGC; COFS3; ERCM2
NCBI Protein Information
DNA repair protein complementing XP-G cells; XPG-complementing protein; DNA excision repair protein ERCC-5; xeroderma pigmentosum, complementation group G
UniProt Protein Name
DNA repair protein complementing XP-G cells
UniProt Gene Name
ERCC5
UniProt Synonym Gene Names
ERCM2; XPG; XPGC
UniProt Entry Name
ERCC5_HUMAN
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Product Notes
The ERCC5/XPG ercc5 (Catalog #AAA211499) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ERCC5/XPG Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERCC5/XPG can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ERCC5/XPG ercc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERCC5/XPG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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