Rabbit anti-Human ERK1 Polyclonal Antibody | anti-ERK1 antibody

Rabbit anti-ERK1 Antibody, Affinity Purified

Cat. Num. AAA211743

• Gene Names: MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• Reactivity: Human
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: ERK1, Antibody; Rabbit anti-ERK1 Antibody, Affinity Purified; HS44KDAP; MAPK 1; p44-MAPK; P44MAPK; p44-ERK1; P44ERK1; mitogen-activated protein kinase 3; microtubule-associated protein 2 kinase; MAPK 3; MAP kinase isoform p44; MAP kinase 3; HUMKER1A; PRKM3; extracellular signal-related kinase 1; extracellular signal-regulated kinase 1; ERT2; ERK-1; ERK1; insulin-stimulated MAP2 kinase; Mitogen-activated protein kinase 3; anti-ERK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
• Concentration: 200ug/ml (varies by lot)

• Applicable Applications for anti-ERK1 antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 329 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human ERK1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ERK1 antibody AAA211743 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human ERK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ERK1 antibody AAA211743 used for IP at 3 ug/mg lysate. For blotting immunoprecipitated ERK1, AAA211743 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.)

IHC (Immunohistochemistry)

(Detection of human ERK1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ERK1 (Cat. No. AAA211743 lot 1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)

Related Product Information for anti-ERK1 antibody

Extracellular signal-regulated kinase 1 (ERK1/p44), also known as mitogen-activated protein kinase 3 (MAPK3), and extracellular signal-regulated kinase 2 (ERK2/p42), also known as mitogen-activated protein kinase 1 (MAPK1), are part of a signal transduction pathway that begins with the small GTPase Ras and continues through a cascade of protein kinases that include Raf and MEK. Activation of this signaling pathway influences many cellular processes such as proliferation, differentiation and survival. ERK1 is very similar in sequence to ERK2/p42 and the activities of both ERK1 and ERK2 are usually considered together as total ERK activity. More recent evidence has shown that ERK1 and ERK2 are not functionally redundant and may have very different and specific roles.

Product Categories/Family for anti-ERK1 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 91718899
• NCBI GeneID: 5595
• NCBI Accession #: NP_002737.2
• NCBI GenBank Nucleotide #: NM_002746.2
• UniProt Accession #: P27361; Q8NHX1; A8CZ58; B0LPG3
• Molecular Weight: 40,088 Da
• NCBI Official Full Name: mitogen-activated protein kinase 3 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 3
• NCBI Official Symbol: MAPK3
• NCBI Official Synonym Symbols: ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• NCBI Protein Information: mitogen-activated protein kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase 3
• UniProt Gene Name: MAPK3
• UniProt Synonym Gene Names: ERK1; PRKM3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK
• UniProt Entry Name: MK03_HUMAN