WB (Western Blot)
((1.5ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp. ) were analyzed in parallel in this Western blot labelled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel).)
Goat ERK3/MAPK6 Polyclonal Antibody | anti-Mapk6 antibody
Goat anti-ERK3/MAPK6 Antibody
Gene Names
MAPK6; ERK3; PRKM6; p97MAPK; HsT17250
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
Immunoprecipitation, Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ERK3/MAPK6, Antibody; Goat anti-ERK3/MAPK6 Antibody; MAPK6; mitogen-activated protein kinase 6; ERK3; HsT17250; PRKM6; p97MAPK; ERK-3; MAP kinase 6; MAP kinase isoform p97; MAPK 6; extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97; p97-MAPK; protein kinase, mitogen-activate; MAPK6 antibody; mitogen-activated protein kinase 6 antibody; ERK3 antibody; HsT17250 antibody; PRKM6 antibody; p97MAPK antibody; ERK-3 antibody; MAP kinase 6 antibody; MAP kinase isoform p97 antibody; MAPK 6 antibody; extracellular signal-regulated kinase 3 antibody; extracellular signal-regulated kinase; p97 antibody; p97-MAPK antibody; protein kinase; mitogen-activated 5 antibody; mitogen-activated 6 antibody; ERK3 / MAPK6; anti-Mapk6 antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
HSPVGSPLKSIQ
Sequence Length
721
Applicable Applications for anti-Mapk6 antibody
IP (Immunoprecipitation), WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-HSPVGSPLKSIQ, from the internal region (near C Terminus) of the protein sequence according to NP_002739.1.
Epitope
Internal region (near C Terminus)
Note
Reported variants represent identical protein (NP_056621.4; NP_081694.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
WB (Western Blot)
((1.5ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp. ) were analyzed in parallel in this Western blot labelled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel).)
WB (Western Blot)
((1.5ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp. ) were analyzed in parallel in this Western blot labelled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel).)
WB (Western Blot)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with (0.5ug/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.)
WB (Western Blot)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with (0.5ug/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
82,681 Da
NCBI Official Full Name
mitogen-activated protein kinase 6
NCBI Official Synonym Full Names
mitogen-activated protein kinase 6
NCBI Official Symbol
MAPK6
NCBI Official Synonym Symbols
ERK3; PRKM6; p97MAPK; HsT17250
NCBI Protein Information
mitogen-activated protein kinase 6
UniProt Protein Name
Mitogen-activated protein kinase 6
UniProt Gene Name
MAPK6
UniProt Synonym Gene Names
ERK3; PRKM6; MAP kinase 6; MAPK 6; ERK-3; p97-MAPK
UniProt Entry Name
MK06_HUMAN
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Product Notes
The Mapk6 mapk6 (Catalog #AAA61660) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ERK3/MAPK6 Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ERK3/MAPK6 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the Mapk6 mapk6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HSPVGSPLKS IQ. It is sometimes possible for the material contained within the vial of "ERK3/MAPK6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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