IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit Phospho-ERK3-S189 Polyclonal Antibody | anti-MAPK6 antibody
Phospho-ERK3-S189 Antibody
Gene Names
MAPK6; ERK3; PRKM6; p97MAPK; HsT17250
Reactivity
HumanPredicted Reactivity: Mouse, Rat, Chicken
Applications
Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-ERK3-S189, Antibody; Phospho-ERK3-S189 Antibody; Mitogen-activated protein kinase 6; MAP kinase 6; MAPK 6; Extracellular signal-regulated kinase 3; ERK-3; MAP kinase isoform p97; p97-MAPK; MAPK6; ERK3; PRKM6; anti-MAPK6 antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Mouse, Rat, Chicken
Predicted Reactivity: Mouse, Rat, Chicken
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Phospho-ERK3-S189 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S189 of human ERK3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
721
Applicable Applications for anti-MAPK6 antibody
IHC (Immunohistochemistry), ELISA
Function
Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).
Cellular Location
Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following interaction with MAPKAPK5
Tissue Location
Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-MAPK6 antibody
ERK3 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
Product Categories/Family for anti-MAPK6 antibody
References
Rai, R., et al., Oral Oncol. 40(7):705-712 (2004).
Coulombe, P., et al., Mol. Cell. Biol. 24(14):6140-6150 (2004).
Coulombe, P., et al., Mol. Cell. Biol. 23(13):4542-4558 (2003).
Julien, C., et al., J. Biol. Chem. 278(43):42615-42624 (2003).
Robinson, M.J., et al., J. Biol. Chem. 277(7):5094-5100 (2002).
Coulombe, P., et al., Mol. Cell. Biol. 24(14):6140-6150 (2004).
Coulombe, P., et al., Mol. Cell. Biol. 23(13):4542-4558 (2003).
Julien, C., et al., J. Biol. Chem. 278(43):42615-42624 (2003).
Robinson, M.J., et al., J. Biol. Chem. 277(7):5094-5100 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
mitogen-activated protein kinase 6
NCBI Official Synonym Full Names
mitogen-activated protein kinase 6
NCBI Official Symbol
MAPK6
NCBI Official Synonym Symbols
ERK3; PRKM6; p97MAPK; HsT17250
NCBI Protein Information
mitogen-activated protein kinase 6
UniProt Protein Name
Mitogen-activated protein kinase 6
UniProt Gene Name
MAPK6
UniProt Synonym Gene Names
ERK3; PRKM6; MAP kinase 6; MAPK 6; ERK-3; p97-MAPK
UniProt Entry Name
MK06_HUMAN
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Product Notes
The MAPK6 mapk6 (Catalog #AAA289488) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-ERK3-S189 Antibody reacts with Human Predicted Reactivity: Mouse, Rat, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-ERK3-S189 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the MAPK6 mapk6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-ERK3-S189, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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