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product-image-AAA211953_WB13.jpg WB (Western Blot) (Detection of human ERK5 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 ug) and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-ERK5 antibody AAA211953 (lot AAA211953) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

Rabbit anti-Human ERK5 Polyclonal Antibody | anti-ERK5 antibody

Rabbit anti-ERK5 Antibody, Affinity Purified

Gene Names
MAPK7; BMK1; ERK4; ERK5; PRKM7
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ERK5, Antibody; Rabbit anti-ERK5 Antibody, Affinity Purified; ERK5; mitogen-activated protein kinase 7; MAPK 7; MAP kinase 7; extracellular-signal-regulated kinase 5; PRKM7; Extracellular signal-regulated kinase 5; ERK4; BMK1 kinase; BMK-1; BMK1; big MAP kinase 1; ERK-5; Mitogen-activated protein kinase 7; anti-ERK5 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-ERK5 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 766 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human ERK5 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 ug) and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-ERK5 antibody AAA211953 (lot AAA211953) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

product-image-AAA211953_WB13.jpg WB (Western Blot) (Detection of human ERK5 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 ug) and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-ERK5 antibody AAA211953 (lot AAA211953) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IP (Immunoprecipitation)

(Detection of human ERK5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ERK5 antibody AAA211953 (lot AAA211953-1) used for IP at 3 ug/mg lysate. ERK5 was also immunoprecipitated by rabbit anti-ERK5 antibody which recognizes an upstream epitope. For blotting immunoprecipitated ERK5, AAA211953 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.)

product-image-AAA211953_IP15.jpg IP (Immunoprecipitation) (Detection of human ERK5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ERK5 antibody AAA211953 (lot AAA211953-1) used for IP at 3 ug/mg lysate. ERK5 was also immunoprecipitated by rabbit anti-ERK5 antibody which recognizes an upstream epitope. For blotting immunoprecipitated ERK5, AAA211953 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.)
Related Product Information for anti-ERK5 antibody
ERK5 (extracellular signal-regulated kinase 5) is a member of the MAP kinase family. ERK5 is also known as MAPK7 (mitogen-activated protein kinase 7). ERK5 is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors [taken from NCBI Entrez Gene (GeneID: 5598)].
Product Categories/Family for anti-ERK5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88,386 Da
NCBI Official Full Name
mitogen-activated protein kinase 7 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase 7
NCBI Official Symbol
MAPK7
NCBI Official Synonym Symbols
BMK1; ERK4; ERK5; PRKM7
NCBI Protein Information
mitogen-activated protein kinase 7; BMK-1; ERK-5; MAPK 7; BMK1 kinase; MAP kinase 7; big MAP kinase 1; extracellular signal-regulated kinase 5; extracellular-signal-regulated kinase 5
UniProt Protein Name
Mitogen-activated protein kinase 7
UniProt Gene Name
MAPK7
UniProt Synonym Gene Names
BMK1; ERK5; PRKM7; MAP kinase 7; MAPK 7; BMK-1; ERK-5
UniProt Entry Name
MK07_HUMAN

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Product Notes

The ERK5 mapk7 (Catalog #AAA211953) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ERK5 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERK5 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ERK5 mapk7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERK5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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