Rabbit ERK5/MAPK7 Polyclonal Antibody | anti-MAPK7 antibody

Anti-ERK5/MAPK7 Antibody

Cat. Num. AAA125672

• Gene Names: MAPK7; BMK1; ERK4; ERK5; PRKM7
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: ERK5/MAPK7, Antibody; Anti-ERK5/MAPK7 Antibody; MAPK7; BMK1; ERK5; PRKM7; Mitogen-activated protein kinase 7; MAP kinase 7; MAPK 7; EC 2. 7. 11. 24; Big MAP kinase 1; BMK-1; Extracellular signal-regulated kinase 5; ERK-5; mitogen-activated protein kinase 7; anti-MAPK7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for ERK5/MAPK7 detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

• Applicable Applications for anti-MAPK7 antibody: ELISA (Direct ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived human ERK5/MAPK7 recombinant protein (Position: K22-P816).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

IHC (Immunohistochemisry)

(Figure 3. IHC analysis of ERK5/MAPK7 using anti-ERK5/MAPK7 antibody (AAA125672).ERK5/MAPK7 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ERK5/MAPK7 Antibody (AAA125672) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohiostchemistry)

(Figure 2. IHC analysis of ERK5/MAPK7 using anti-ERK5/MAPK7 antibody (AAA125672).ERK5/MAPK7 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ERK5/MAPK7 Antibody (AAA125672) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot)

(Figure 1. Western blot analysis of ERK5/MAPK7 using anti-ERK5/MAPK7 antibody (AAA125672).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human Hek293 whole cell lysatesLane 3: human Hela whole cell lysatesLane 4: monkey Cos-7 whole cell lysatesLane 5: rat brain tissue lysatesLane 6: mouse brain tissue lysatesLane 7: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ERK5/MAPK7 antigen affinity purified polyclonal antibody (Catalog # AAA125672) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ERK5/MAPK7 at approximately 115-120KD. The expected band size for ERK5/MAPK7 is at 88KD.)

Related Product Information for anti-MAPK7 antibody

Mitogen-activated protein kinase 7 also known as MAP kinase 7 is an enzyme that in humans is encoded by the MAPK7 gene. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported.

References

1. Hayashi, M., Kim, S. -W., Imanaka-Yoshida, K., Yoshida, T., Abel, E. D., Eliceiri, B., Yang, Y., Ulevitch, R. J., Lee, J. -D. Targeted deletion of BMK1/ERK5 in adult mice perturbs vascular integrity and leads to endothelial failure. J. Clin. Invest. 113: 1138-1148, 2004.
2. Lee, J. -D., Ulevitch, R. J., Han, J. Primary structure of BMK1: a new mammalian MAP kinase. Biochem. Biophys. Res. Commun. 213: 715-724, 1995.
3. Purandare, S. M., Lee, J. -D., Patel, P. I. Assignment of big MAP kinase (PRKM7) to human chromosome 17 band p11. 2 with somatic cell hybrids. Cytogenet. Cell Genet. 83: 258-259, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 20986497
• NCBI GeneID: 5598
• NCBI Accession #: NP_002740.2
• NCBI GenBank Nucleotide #: NM_002749.3
• UniProt Accession #: Q13164; Q16634; Q59F50; Q6QLU7; Q7L4P4; Q969G1; Q96G51
• Molecular Weight: 88,386 Da
• NCBI Official Full Name: mitogen-activated protein kinase 7 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 7
• NCBI Official Symbol: MAPK7
• NCBI Official Synonym Symbols: BMK1; ERK4; ERK5; PRKM7
• NCBI Protein Information: mitogen-activated protein kinase 7; BMK-1; ERK-5; MAPK 7; BMK1 kinase; MAP kinase 7; big MAP kinase 1; extracellular signal-regulated kinase 5; extracellular-signal-regulated kinase 5
• UniProt Protein Name: Mitogen-activated protein kinase 7
• UniProt Gene Name: MAPK7
• UniProt Synonym Gene Names: BMK1; ERK5; PRKM7; MAP kinase 7; MAPK 7; BMK-1; ERK-5
• UniProt Entry Name: MK07_HUMAN