IF (Immunofluorescence)
(AAA328950 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328950 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit ERp29 Polyclonal Antibody | anti-ERP29 antibody
ERp29 Antibody
Gene Names
ERP29; ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA, Immunocytochemistry, Immunofluorescence
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
ERp29, Antibody; ERp29 Antibody; C12orf8; Chromosome 12 open reading frame 8; Endoplasmic reticulum lumenal protein ERp28; Endoplasmic reticulum protein 29; Endoplasmic reticulum protein 29 isoform 1; Endoplasmic reticulum protein ERp29; Endoplasmic reticulum resident protein 28; Endoplasmic reticulum resident protein 29; Endoplasmic reticulum resident protein 31; Epididymis secretory protein Li 107; ERp28; ERP29_HUMAN; ERp31; HEL S 107; PDI-DB; PDIA9; Protein disulfide isomerase family A member 9; anti-ERP29 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
ERp29 Antibody detects endogenous levels of total ERp29.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
53
Applicable Applications for anti-ERP29 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
A synthesized peptide derived from human ERp29, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328950 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328950 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA328950 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328950 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IHC (Immunohiostchemistry)
(AAA328950 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328950 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from NIH/3T3, using ERp29 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from NIH/3T3, using ERp29 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-ERP29 antibody
Function: Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.
Subcellular Location: Endoplasmic reticulum lumen. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue Specificity: Ubiquitous. Mostly expressed in secretory tissues.
Subunit Structure: Homodimer. Part of a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.
Subcellular Location: Endoplasmic reticulum lumen. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue Specificity: Ubiquitous. Mostly expressed in secretory tissues.
Subunit Structure: Homodimer. Part of a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)28kD.
Predicted Molecular Weight: (Calculated)29kDa.
Predicted Molecular Weight: (Calculated)29kDa.
NCBI Official Full Name
endoplasmic reticulum resident protein 29 isoform 2
NCBI Official Synonym Full Names
endoplasmic reticulum protein 29
NCBI Official Symbol
ERP29
NCBI Official Synonym Symbols
ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
NCBI Protein Information
endoplasmic reticulum resident protein 29
UniProt Protein Name
Endoplasmic reticulum resident protein 29
UniProt Gene Name
ERP29
UniProt Synonym Gene Names
C12orf8; ERP28; ERp29; ERp28; ERp31
UniProt Entry Name
ERP29_HUMAN
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Product Notes
The ERP29 erp29 (Catalog #AAA328950) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERp29 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's ERp29 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the ERP29 erp29 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERp29, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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