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FCM/FACS (Flow Cytometry)
(ERP29 Antibody (Center) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human ERP29 Polyclonal Antibody | anti-ERP29 antibody
ERP29 Antibody (Center)
Gene Names
ERP29; ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ERP29, Antibody; ERP29 Antibody (Center); Endoplasmic reticulum resident protein 29; ERp29; Endoplasmic reticulum resident protein 28; ERp28; Endoplasmic reticulum resident protein 31; ERp31; ERP29; C12orf8; ERP28; anti-ERP29 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ERP29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-179 amino acids from the Central region of human ERP29.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
151-179
Sequence Length
53
Applicable Applications for anti-ERP29 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ERP29 Antibody (Center) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ERP29 Antibody (Center) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ERP29 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ERP29 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of ERP29 (arrow) using rabbit polyclonal ERP29 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERP29 gene (Lane 2).)
WB (Western Blot)
(Western blot analysis of ERP29 (arrow) using rabbit polyclonal ERP29 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERP29 gene (Lane 2).)
WB (Western Blot)
(Western blot analysis of ERP29 Antibody (Center) in A2058,A375,MCF7,NCI-H460,Y79 cell line lysates (35ug/lane). ERP29 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of ERP29 Antibody (Center) in A2058,A375,MCF7,NCI-H460,Y79 cell line lysates (35ug/lane). ERP29 (arrow) was detected using the purified Pab.)
Related Product Information for anti-ERP29 antibody
ERP29 shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
Product Categories/Family for anti-ERP29 antibody
References
Bambang,I.F., et.al., Exp. Cell Res. 315 (11), 1964-1974 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28993
NCBI Official Full Name
endoplasmic reticulum resident protein 29 isoform 2
NCBI Official Synonym Full Names
endoplasmic reticulum protein 29
NCBI Official Symbol
ERP29
NCBI Official Synonym Symbols
ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
NCBI Protein Information
endoplasmic reticulum resident protein 29
UniProt Protein Name
Endoplasmic reticulum resident protein 29
UniProt Gene Name
ERP29
UniProt Synonym Gene Names
C12orf8; ERP28; ERp29; ERp28; ERp31
UniProt Entry Name
ERP29_HUMAN
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Product Notes
The ERP29 erp29 (Catalog #AAA283879) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 151-179. The ERP29 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERP29 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the ERP29 erp29 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERP29, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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