IHC (Immunohiostchemistry)
(Anti-ERp57 antibody, AAA45045, IHC(P)IHC(P): Human Placenta Tissue)
Rabbit ERp57 Polyclonal Antibody | anti-PDIA3 antibody
Anti-ERp57 antibody
Gene Names
PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
ERp57, Antibody; Anti-ERp57 antibody; Protein disulfide-isomerase A3; protein disulfide isomerase family A, member 3; 58 kDa glucose regulated protein antibody; 58 kDa glucose-regulated protein antibody; 58 kDa microsomal protein antibody; Disulfide isomerase ER 60 antibody; Disulfide isomerase ER-60 antibody; Endoplasmic reticulum resident protein 57 antibody; Endoplasmic reticulum resident protein 60 antibody; ER p57 antibody; ER protein 57 antibody; ER protein 60 antibody; ERp 57 antibody; ERp57 antibody; ERp60 antibody; ERp61 antibody; Glucose Regulated Protein 58 Kd antibody; GRP 57 antibody; GRP 58 antibody; GRP57 antibody; GRP58 antibody; HsT17083 antibody; P58 antibody; PDIA 3 antibody; PDIA3 antibody; PDIA3_HUMAN antibody; Phospholipase C alpha antibody; PI PLC antibody; Protein disulfide isomerase A3 antibody; Protein disulfide isomerase family A member 3 antibody; Protein disulfide-isomerase A3 antibody; anti-PDIA3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
505
Applicable Applications for anti-PDIA3 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ERp57 (172-188aa LKAASNLRDNYRFAHTN), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti-ERp57 antibody, AAA45045, IHC(P)IHC(P): Human Placenta Tissue)
IHC (Immunohiostchemistry)
(Anti-ERp57 antibody, AAA45045, IHC(P)IHC(P): Human Placenta Tissue)
WB (Western Blot)
(Anti-ERp57 antibody, AAA45045, Western blottingLane 1: SMMC Cell LysateLane 2: A549 Cell LysateLane 3: U87 Cell LysateLane 4: HELA Cell LysateLane 5: MCF-7 Cell Lysate)
WB (Western Blot)
(Anti-ERp57 antibody, AAA45045, Western blottingLane 1: SMMC Cell LysateLane 2: A549 Cell LysateLane 3: U87 Cell LysateLane 4: HELA Cell LysateLane 5: MCF-7 Cell Lysate)
Related Product Information for anti-PDIA3 antibody
Description: Rabbit IgG polyclonal antibody for Protein disulfide-isomerase A3(PDIA3) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: PDIA3(Protein disulfide isomerase family A, member 3), also called GRP58, Erp57 or ER60, is an isomerase enzyme, mapped on 15q15.3. The PDIA3 protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex(MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Koivunen et al. showed that PDIA3 could not substitute for the beta subunit of PDI. When coexpressed with alpha prolyl 4-hydroxylase, PDIA3 did not form prolyl 4-hydroxylase tetramers, nor did it have prolyl 4-hydroxylase activity. Hirano et al. expressed human PDIA3 and found that the protein had a thiol-dependent reductase activity. They showed that the expression level of PDIA3 is increased after oncogenic transformation of normal rat kidney cells and NIH 3T3 cells.
Background: PDIA3(Protein disulfide isomerase family A, member 3), also called GRP58, Erp57 or ER60, is an isomerase enzyme, mapped on 15q15.3. The PDIA3 protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex(MHC) class I peptide-loading complex, which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Koivunen et al. showed that PDIA3 could not substitute for the beta subunit of PDI. When coexpressed with alpha prolyl 4-hydroxylase, PDIA3 did not form prolyl 4-hydroxylase tetramers, nor did it have prolyl 4-hydroxylase activity. Hirano et al. expressed human PDIA3 and found that the protein had a thiol-dependent reductase activity. They showed that the expression level of PDIA3 is increased after oncogenic transformation of normal rat kidney cells and NIH 3T3 cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
56,782 Da
NCBI Official Full Name
Protein disulfide-isomerase A3
NCBI Official Synonym Full Names
protein disulfide isomerase family A, member 3
NCBI Official Symbol
PDIA3
NCBI Official Synonym Symbols
P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
NCBI Protein Information
protein disulfide-isomerase A3; ER protein 57; ER protein 60; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; epididymis secretory protein Li 269; protein disulfide isomerase-associated 3; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 93n
UniProt Protein Name
Protein disulfide-isomerase A3
UniProt Gene Name
PDIA3
UniProt Synonym Gene Names
ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60
UniProt Entry Name
PDIA3_HUMAN
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Product Notes
The PDIA3 pdia3 (Catalog #AAA45045) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ERp57 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ERp57 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PDIA3 pdia3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERp57, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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