WB (Western Blot)
(Western blot analysis of extracts from 293, using ERp72 antibody. Lane 1 was treated with the blocking peptide.)
Rabbit ERp72 Polyclonal Antibody | anti-ERp72 antibody
ERp72 Antibody
Gene Names
PDIA4; ERP70; ERP72; ERp-72
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ERp72, Antibody; ERp72 Antibody; Calcium binding protein intestinal related; Endoplasmic reticulum resident protein 70; Endoplasmic reticulum resident protein 72; EPR70; EPR72; ER protein 70; ER protein 72; ERP 70; ERp 72; ERp-72; ERp70; ERp72; PDIA 4; Pdia4; PDIA4_HUMAN; PDIR; Protein disulfide isomerase A4; Protein disulfide isomerase associated 4; Protein disulfide isomerase family A member 4; protein disulfide isomerase related protein (calcium-binding protein, intestinal-related); Protein disulfide isomerase related protein; Protein disulfide-isomerase A4; Protein ERp 72; Protein ERp72; anti-ERp72 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ERp72 antibody detects endogenous levels of ERp72.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
645
Applicable Applications for anti-ERp72 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ERp72.
Subcellular Location
Endoplasmic Reticulum Lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Predicted Cross Reactivity
Horse, Rabbit, Chicken
Similarity
Horse (92%), Rabbit (92%), Chicken (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from 293, using ERp72 antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from 293, using ERp72 antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts of HepG2 cells, using ERp72 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of HepG2 cells, using ERp72 antibody.)
IHC (Immunohistochemistry)
(AAA322940 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA322940 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-ERp72 antibody
Function: Was originally thought to be a deoxycytidine kinase.
Subunit Structure: Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.
Similarity: Belongs to the protein disulfide isomerase family.
Subunit Structure: Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.
Similarity: Belongs to the protein disulfide isomerase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 72 kDa
Predicted: 73 kDa
Predicted: 73 kDa
NCBI Official Full Name
protein disulfide-isomerase A4
NCBI Official Synonym Full Names
protein disulfide isomerase family A member 4
NCBI Official Symbol
PDIA4
NCBI Official Synonym Symbols
ERP70; ERP72; ERp-72
NCBI Protein Information
protein disulfide-isomerase A4
UniProt Protein Name
Protein disulfide-isomerase A4
UniProt Gene Name
PDIA4
UniProt Synonym Gene Names
ERP70; ERP72; ER protein 70; ERp70; ER protein 72; ERp-72; ERp72
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Product Notes
The ERp72 pdia4 (Catalog #AAA322940) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERp72 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ERp72 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ERp72 pdia4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERp72, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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