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IF (Immunofluorescence)
(Immunofluorescence staining of PDIA4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PDIA4 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit anti-Human ERP72 Monoclonal Antibody | anti-PDIA4 antibody
Recombinant Anti-ERP72 Antibody, Rabbit Monoclonal
Gene Names
PDIA4; ERP70; ERP72; ERp-72
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot
Synonyms
ERP72, Antibody; Recombinant Anti-ERP72 Antibody, Rabbit Monoclonal; ERP72/PDIA4 Antibody, Rabbit MAb; protein disulfide isomerase family A, member 4; Anti-ERp-72 Antibody; Anti-ERP70 Antibody; Anti-ERP72 Antibody; anti-PDIA4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
020
Specificity
Human ERP72/PDIA4
Form/Format
0.2um filtered solution in PBS
Applicable Applications for anti-PDIA4 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
Recombinant Human ERP72/PDIA4 Protein
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ERP72/PDIA4 (rh ERP72/PDIA4).
Preparation and Storage
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of PDIA4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PDIA4 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of PDIA4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PDIA4 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human PDIA4 expression on HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human PDIA4, t)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human PDIA4 expression on HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human PDIA4, t)
WB (Western Blot)
(Anti-PDIA4 rabbit monoclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: NIH-3T3 Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:73 kDaObserved band size:73 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-PDIA4 rabbit monoclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: NIH-3T3 Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:73 kDaObserved band size:73 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(PDIA4 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-PDIA4 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-PDIA4 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 73 kDaObserved band size: 73 kDa)
IP (Immunoprecipitation)
(PDIA4 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-PDIA4 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-PDIA4 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 73 kDaObserved band size: 73 kDa)
Related Product Information for anti-PDIA4 antibody
ERP72, also known as PDIA4, is an endoplasmic reticulum luminal protein which belongs to the protein disulfide isomerase family. ERP72 is a stress protein and participates in the catalysis of protein-S-S-bond rearrangement. Both PDIA4 and PDIA3 function as proteases, protein disulfide isomerases, phospholipases or an arrangement of these. ERP72 compose part of a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.
References
Tsai YC, et al. (2012) Functional proteomics establishes the interaction of SIRT7 with chromatin remodeling complexes and expands its role in regulation of RNA polymerase I transcription. Mol Cell Proteomics. 11(5):60-76.Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40.Vinayagam A, et al. (2011) A directed protein interaction network for investigating intracellular signal transduction. Sci Signal. 4(189):rs8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Protein disulfide-isomerase A4
NCBI Official Synonym Full Names
protein disulfide isomerase family A, member 4
NCBI Official Symbol
PDIA4
NCBI Official Synonym Symbols
ERP70; ERP72; ERp-72
NCBI Protein Information
protein disulfide-isomerase A4; ER protein 70; ER protein 72; protein disulfide isomerase-associated 4; endoplasmic reticulum resident protein 70; endoplasmic reticulum resident protein 72; protein disulfide isomerase related protein (calcium-binding prot
UniProt Protein Name
Protein disulfide-isomerase A4
UniProt Gene Name
PDIA4
UniProt Synonym Gene Names
ERP70; ERP72; ER protein 70; ERp70; ER protein 72; ERp-72; ERp72
UniProt Entry Name
PDIA4_HUMAN
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Product Notes
The PDIA4 pdia4 (Catalog #AAA255668) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-ERP72 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERP72 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the PDIA4 pdia4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERP72, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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