WB (Western Blot)
(Western blot analysis of extracts from A431 cells, using ESE1 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit ESE1 Polyclonal Antibody | anti-ELF3 antibody
ESE1 Antibody
Gene Names
ELF3; ERT; ESX; EPR-1; ESE-1
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
ESE1, Antibody; ESE1 Antibody; E74 like factor 3; E74 like factor 3 ets domain transcription factor; E74 like factor 3 ets domain transcription factor epithelial specific; E74 like factor 3 ETS domain transcription factor serine box epithelial specific; E74-like factor 3; Elf3; ELF3_HUMAN; Epithelial restricted with serine box; Epithelial-restricted with serine box; Epithelium restricted Ets protein ESX; Epithelium specific Ets factor 1; Epithelium specific Ets transcription factor 1; Epithelium-restricted Ets protein ESX; Epithelium-specific Ets transcription factor 1; EPR 1; EPR1; ERT; ESE-1; ESX; Ets domain transcription factor serine box; Ets domain transcription factor serine box epithelial specific; Ets transcription factor; ETS-related transcription factor Elf-3; jen; MGC139318; anti-ELF3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
ESE1 Antibody detects endogenous levels of total ESE1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
371
Applicable Applications for anti-ELF3 antibody
ELISA
Immunogen
A synthesized peptide derived from human ESE1
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from A431 cells, using ESE1 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from A431 cells, using ESE1 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from DU145 cells, using ESE1 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 55 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from DU145 cells, using ESE1 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 55 kDa.)
Related Product Information for anti-ELF3 antibody
Function: Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development (By similarity).
Subcellular Location: Cytoplasm. Nucleus. Note: Localizes to the cytoplasm where it has been shown to transform MCF-12A mammary epithelial cells via a novel cytoplasmic mechanism. Also transiently expressed and localized to the nucleus where it induces apoptosis in non-transformed breast epithelial cells MCF-10A and MCF-12A via a transcription-dependent mechanism.
Tissue Specificity: Expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin.
Subunit Structure: Interacts with TBP. Interacts with CREBBP and EP300; these act as transcriptional coactivators of ELF3 and positively modulate its function. Interacts with XRCC5/KU86 and XRCC6/KU70; these inhibit the ability of ELF3 to bind DNA and negatively modulate its transcriptional activity. Associated with CLND7 and POU2F3.
Similarity: the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.Belongs to the ETS family.
Subcellular Location: Cytoplasm. Nucleus. Note: Localizes to the cytoplasm where it has been shown to transform MCF-12A mammary epithelial cells via a novel cytoplasmic mechanism. Also transiently expressed and localized to the nucleus where it induces apoptosis in non-transformed breast epithelial cells MCF-10A and MCF-12A via a transcription-dependent mechanism.
Tissue Specificity: Expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin.
Subunit Structure: Interacts with TBP. Interacts with CREBBP and EP300; these act as transcriptional coactivators of ELF3 and positively modulate its function. Interacts with XRCC5/KU86 and XRCC6/KU70; these inhibit the ability of ELF3 to bind DNA and negatively modulate its transcriptional activity. Associated with CLND7 and POU2F3.
Similarity: the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.Belongs to the ETS family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)42kD, 55kD.
Predicted Molecular Weight: (Calculated)41kDa.
Predicted Molecular Weight: (Calculated)41kDa.
NCBI Official Full Name
ETS-related transcription factor Elf-3
NCBI Official Synonym Full Names
E74 like ETS transcription factor 3
NCBI Official Symbol
ELF3
NCBI Official Synonym Symbols
ERT; ESX; EPR-1; ESE-1
NCBI Protein Information
ETS-related transcription factor Elf-3
UniProt Protein Name
ETS-related transcription factor Elf-3
UniProt Gene Name
ELF3
UniProt Synonym Gene Names
ESE-1
UniProt Entry Name
ELF3_HUMAN
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Product Notes
The ELF3 elf3 (Catalog #AAA329077) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ESE1 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's ESE1 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the ELF3 elf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ESE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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