Rabbit anti-Human EXOSC2 Polyclonal Antibody | anti-EXOSC2 antibody

Anti-EXOSC2 Antibody, Rabbit Polyclonal

Cat. Num. AAA256389

• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: EXOSC2, Antibody; Anti-EXOSC2 Antibody, Rabbit Polyclonal; EXOSC2 Antibody, Rabbit PAb, Antigen Affinity Purified; exosome component 2; Anti-hRrp4p Antibody; Anti-p7 Antibody; Anti-RRP4 Antibody; Anti-Rrp4p Antibody; anti-EXOSC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human EXOSC2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-EXOSC2 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived Human EXOSC2 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human EXOSC2 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(EXOSC2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-EXOSC2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-EXOSC2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size :33 kDa)

IHC (Immunohistochemisry)

(Immunochemical staining of human EXOSC2 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human EXOSC2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-EXOSC2 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: Hela Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:33 kDa)

Related Product Information for anti-EXOSC2 antibody

Missense mutations in the RNA exosome component 2 (EXOSC2), also known as ribosomal RNA-processing protein 4 (RRP4), were recently identified in two unrelated families with a novel syndrome known as Short stature, Hearing loss, Retinitis pigmentosa, and distinctive Facies (SHRF). EXOSC2 encodes the 'ribosomal RNA-processing protein 4' (RRP4)-one of the core components of the RNA exosome. The RNA exosome is a multiprotein complex that plays key roles in RNA processing and degradation. Mutations in the RNA exosome gene EXOSC2 cause a distinct syndrome with various tissue-specific phenotypes including retinitis pigmentosa, hearing loss, and mild intellectual disability. Retinitis pigmentosa in combination with hearing loss can be a feature of different Mendelian disorders.

References

Yang X, et al. (2020) Genetic and genomic studies of pathogenic exosc2 mutations in the newly described disease shrf implicate the autophagy pathway in disease pathogenesis. Hum Mol Genet 29 (4): 541-553.Morton DJ, et al. (2018) The rna exosome and rna exosome-linked disease. Rna 24 (2): 127-142.Di Donato N, et al. (2016) Mutations in exosc2 are associated with a novel syndrome characterised by retinitis pigmentosa, progressive hearing loss, premature ageing, short stature, mild intellectual disability and distinctive gestalt. J Med Genet 53 (6): 419-425.