FCM/FACS (Flow Cytometry)
(Overlay histogram showing A549 cells stained with AP22333a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22333a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit anti-Human F13A1 Polyclonal Antibody | anti-F13A1 antibody
F13A1 Antibody (N-Term)
Gene Names
F13A1; F13A
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Western Blot
Synonyms
F13A1, Antibody; F13A1 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Coagulation factor XIII A chain; Coagulation factor XIIIa; 2.3.2.13; Protein-glutamine gamma-glutamyltransferase A chain; Transglutaminase A chain; F13A1; F13A; anti-F13A1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Clone Number
RB57788
Specificity
This F13A1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 60-94 amino acids from the human region of human F13A1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-F13A1 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), WB (Western Blot)
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Overlay histogram showing A549 cells stained with AP22333a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22333a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing A549 cells stained with AP22333a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22333a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
WB (Western Blot)
(Anti-F13A1 Antibody (N-Term) at 1:2000 dilution + Human placenta lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-F13A1 Antibody (N-Term) at 1:2000 dilution + Human placenta lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized A549 cells labeling F13A1 with AP22333a at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG (OH191631) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (1186255) at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized A549 cells labeling F13A1 with AP22333a at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG (OH191631) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (1186255) at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).)
Related Product Information for anti-F13A1 antibody
Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl- epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
Product Categories/Family for anti-F13A1 antibody
References
Ichinose A.,et al.Biochemistry 25:6900-6906(1986).Grundmann U.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:8024-8028(1986).Ichinose A.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:5829-5833(1988).Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.Mungall A.J.,et al.Nature 425:805-811(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,267 Da
NCBI Official Full Name
coagulation factor XIII A chain
NCBI Official Synonym Full Names
coagulation factor XIII, A1 polypeptide
NCBI Official Symbol
F13A1
NCBI Official Synonym Symbols
F13A
NCBI Protein Information
coagulation factor XIII A chain; TGase; factor XIIIa; fibrinoligase; FSF, A subunit; coagulation factor XIIIa; transglutaminase A chain; transglutaminase. plasma; fibrin stabilizing factor, A subunit; coagulation factor XIII, A polypeptide; protein-glutam
UniProt Protein Name
Coagulation factor XIII A chain
UniProt Gene Name
F13A1
UniProt Synonym Gene Names
F13A; Coagulation factor XIIIa
UniProt Entry Name
F13A_HUMAN
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Product Notes
The F13A1 f13a1 (Catalog #AAA290972) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The F13A1 Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's F13A1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the F13A1 f13a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "F13A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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