IP (Immunoprecipitation)
(FABP5 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-FABP5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-FABP5 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)
Rabbit anti-Human FABP5 Polyclonal Antibody | anti-FABP5 antibody
Anti-FABP5 Antibody, Rabbit Polyclonal
Gene Names
FABP5; EFABP; KFABP; E-FABP; PAFABP; PA-FABP
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
FABP5, Antibody; Anti-FABP5 Antibody, Rabbit Polyclonal; FABP5/E-FABP Antibody, Rabbit PAb, Antigen Affinity Purified; fatty acid binding protein 5 (psoriasis-associated); Anti-E-FABP Antibody; Anti-EFABP Antibody; Anti-KFABP Antibody; Anti-PA-FABP Antibody; Anti-PAFABP Antibody; anti-FABP5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human FABP5/E-FABP
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-FABP5 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human FABP5/E-FABP Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human FABP5/E-FABP (rh FABP5/E-FABP). FABP5/E-FABP specific IgG was purified by Human FABP5/E-FABP affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(FABP5 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-FABP5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-FABP5 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)
IP (Immunoprecipitation)
(FABP5 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-FABP5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-FABP5 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of FABP5 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human FABP5 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of FABP5 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human FABP5 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
WB (Western Blot)
(Anti-FABP5 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-FABP5 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-FABP5 antibody
Fatty acid-binding protein, also known as Epidermal-type fatty acid-binding protein, Fatty acid-binding protein 5, Psoriasis-associated fatty acid-binding protein homolog, E-FABP and FABP5, is a cytoplasm protein which Belongs to thecalycin superfamily and Fatty-acid binding protein (FABP) family. Fatty acid-binding proteins (FABPs) are postulated to serve as lipid shuttles that solubilize hydrophobic fatty acids and deliver them to appropriate intracellular sites. E-FABP/FABP5 is predominantly expressed in keratinocytes and is overexpressed in the actively proliferating tissue characteristic of psoriasis and wound healing. E-FABP/FABP5 exhibits an important role in binding free fatty acids, as well as regulating lipid metabolism and transport. E-FABP/FABP5 has high specificity for fatty acids. It has highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity of FABP5. E-FABP/FABP5 may be involved in keratinocyte differentiation.
References
Hohoff C., et al., 1999, Biochemistry 38:12229-39 Gutierrez-Gonzalez L.H., et al., 2002, Biochem. J. 364:725-37. Ogawa, E. et al., 2011, J Invest Dermatol. 131 (3):604-12. Ma, X. et al., 2010, Mol Biol Rep. 37 (8):4003-11.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
15,164 Da
NCBI Official Full Name
Fatty acid-binding protein, epidermal
NCBI Official Synonym Full Names
fatty acid binding protein 5 (psoriasis-associated)
NCBI Official Symbol
FABP5
NCBI Official Synonym Symbols
EFABP; KFABP; E-FABP; PAFABP; PA-FABP
NCBI Protein Information
fatty acid-binding protein, epidermal; epidermal-type fatty acid-binding protein; psoriasis-associated fatty acid-binding protein homolog
UniProt Protein Name
Fatty acid-binding protein, epidermal
UniProt Gene Name
FABP5
UniProt Synonym Gene Names
E-FABP; PA-FABP
UniProt Entry Name
FABP5_HUMAN
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Product Notes
The FABP5 fabp5 (Catalog #AAA255594) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FABP5 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FABP5 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the FABP5 fabp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FABP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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