IF (Immunofluorescence)
(Immunofluorescence staining of FAM129B in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human FAM129B polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cell membrane.)
Rabbit anti-Human FAM129B Polyclonal Antibody | anti-FAM129B antibody
Anti-FAM129B Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Synonyms
FAM129B, Antibody; Anti-FAM129B Antibody, Rabbit Polyclonal; FAM129B Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-bA356B19.6 Antibody; Anti-C9orf88 Antibody; Anti-MEG-3 Antibody; Anti-MINERVA Antibody; Anti-OC58 Antibody; OC58,MEG-3,C9orf88,MINERVA,bA356B19.6; anti-FAM129B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human FAM129B
Form/Format
PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-FAM129B antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
E. coli-derived Human FAM129B fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human FAM129B fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of FAM129B in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human FAM129B polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cell membrane.)
IF (Immunofluorescence)
(Immunofluorescence staining of FAM129B in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human FAM129B polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cell membrane.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human FAM129B in human stomach with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human FAM129B in human stomach with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human FAM129B in human placenta with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human FAM129B in human placenta with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
References
Lee JH, et al. (2016) Fam129b activates ras and promotes aerobic glycolysis. Cell Cycle 15 (11): 1391-1392.Ji H, et al. (2016) Egfr phosphorylates fam129b to promote ras activation. Proc Natl Acad Sci U S A 113 (3): 644-649.
Similar Products
Product Notes
The FAM129B (Catalog #AAA258506) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FAM129B Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FAM129B can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the FAM129B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAM129B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.