WB (Western Blot)
(Anti-FANCA Antibody - Western Blot. Western blot of affinity purified anti-FANCA antibody shows detection of a band at ~133 kD (arrowhead) corres-ponding to FANCA in HeLa whole cell lysates. The identity of the lower molecular weight bands is unknown but may represent breakdown products. Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:1500. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye800 fluorescence images were captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
Rabbit FANCA Polyclonal Antibody | anti-FANCA antibody
Rabbit Polyclonal to Human FANCA
Gene Names
FANCA; FA; FA1; FAA; FAH; FA-H; FACA; FANCH
Reactivity
Chimpanzee, Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
FANCA, Antibody; Rabbit Polyclonal to Human FANCA; Anti-FANCA Antibody (aa995-1009) IHC-plus; FANCA; FA1; FACA; FA-H; Fanconi anemia group A protein; Fanconi anemia; type 1; FA; Protein FACA; FAA; FANCH; Human FANCA; anti-FANCA antibody
Host
Rabbit
Reactivity
Chimpanzee, Human
Clonality
Polyclonal
Specificity
Amino acids 995-1009 of human FANCA protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
1455
Applicable Applications for anti-FANCA antibody
ELISA, WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Synthetic peptide from human FANCA.
Immunogen Type
Synthetic peptide
Immunogen
FANCA antibody was raised against synthetic peptide from human FANCA.
Antigen Modification
aa995-1009
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-FANCA Antibody - Western Blot. Western blot of affinity purified anti-FANCA antibody shows detection of a band at ~133 kD (arrowhead) corres-ponding to FANCA in HeLa whole cell lysates. The identity of the lower molecular weight bands is unknown but may represent breakdown products. Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:1500. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye800 fluorescence images were captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
WB (Western Blot)
(Anti-FANCA Antibody - Western Blot. Western blot of affinity purified anti-FANCA antibody shows detection of a band at ~133 kD (arrowhead) corres-ponding to FANCA in HeLa whole cell lysates. The identity of the lower molecular weight bands is unknown but may represent breakdown products. Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:1500. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye800 fluorescence images were captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
WB (Western Blot)
(Anti-FANCA Antibody - Western Blot. Western blot of affinity purified anti-FANCA antibody shows detection of FANCA only in FANCA transfected GM6914 cell lysates. No staining is seen in lysates prepared from FANCA (-/-) cells in the absence of FANCA transfection. Modified from Smogorzewska et al (2007) Cell 129, 289-301.)
WB (Western Blot)
(Anti-FANCA Antibody - Western Blot. Western blot of affinity purified anti-FANCA antibody shows detection of FANCA only in FANCA transfected GM6914 cell lysates. No staining is seen in lysates prepared from FANCA (-/-) cells in the absence of FANCA transfection. Modified from Smogorzewska et al (2007) Cell 129, 289-301.)
IHC (Immunohistochemistry)
(Anti-FANCA antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-FANCA antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
159,083 Da
NCBI Official Full Name
Fanconi anemia group A protein isoform a
NCBI Official Synonym Full Names
Fanconi anemia, complementation group A
NCBI Official Symbol
FANCA
NCBI Official Synonym Symbols
FA; FA1; FAA; FAH; FA-H; FACA; FANCH
NCBI Protein Information
Fanconi anemia group A protein; Fanconi anemia, type 1; Fanconi anemia, complementation group H
UniProt Protein Name
Fanconi anemia group A protein
UniProt Gene Name
FANCA
UniProt Synonym Gene Names
FAA; FACA; FANCH; Protein FACA
UniProt Entry Name
FANCA_HUMAN
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Product Notes
The FANCA fanca (Catalog #AAA50691) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human FANCA reacts with Chimpanzee, Human and may cross-react with other species as described in the data sheet. AAA Biotech's FANCA can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the FANCA fanca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FANCA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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