IF (Immunofluorescence)
(Confocal immunofluorescent analysis of FAS Antibody (Center) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human FAS Polyclonal Antibody | anti-FAS antibody
FAS Antibody (Center)
Gene Names
FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Reactivity
Human
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FAS, Antibody; FAS Antibody (Center); Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD95; FAS; APT1; FAS1; TNFRSF6; anti-FAS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-211 amino acids from the Central region of human FAS.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
185-211
Sequence Length
220
Applicable Applications for anti-FAS antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of FAS Antibody (Center) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of FAS Antibody (Center) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Western blot analysis of FAS (arrow) using rabbit polyclonal FAS Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FAS gene.)
WB (Western Blot)
(Western blot analysis of FAS (arrow) using rabbit polyclonal FAS Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FAS gene.)
WB (Western Blot)
(Western blot analysis of FAS Antibody (Center) in T47D cell line lysates (35ug/lane).FAS (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of FAS Antibody (Center) in T47D cell line lysates (35ug/lane).FAS (arrow) was detected using the purified Pab.)
Related Product Information for anti-FAS antibody
FAS is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells.
Product Categories/Family for anti-FAS antibody
References
Feig,C., et.al., EMBO J. 26 (1), 221-231 (2007)
Jenkins,M., et.al., J. Biol. Chem. 275 (11), 7988-7993 (2000)
Jenkins,M., et.al., J. Biol. Chem. 275 (11), 7988-7993 (2000)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37732
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 6 isoform 1
NCBI Official Synonym Full Names
Fas cell surface death receptor
NCBI Official Symbol
FAS
NCBI Official Synonym Symbols
APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
NCBI Protein Information
tumor necrosis factor receptor superfamily member 6
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 6
UniProt Gene Name
FAS
UniProt Synonym Gene Names
APT1; FAS1; TNFRSF6
UniProt Entry Name
TNR6_HUMAN
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Product Notes
The FAS fas (Catalog #AAA287619) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 185-211. The FAS Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FAS can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the FAS fas for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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