Rabbit anti-Human FAS Polyclonal Antibody | anti-FAS antibody

FAS Antibody

Cat. Num. AAA307564

• Gene Names: FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: FAS, Antibody; FAS Antibody; FAS; ALPS1A; APO-1; APT1; CD95; anti-FAS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total FAS protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 335

• Applicable Applications for anti-FAS antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human FAS.
• Target Name: FAS
• Preparation and Storage: Store at -20 degree C

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse kidney using FAS antibody at dilution of 1:400 (200x lens).)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded rat spleen using FAS antibody at dilution of 1:400 (200x lens).)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human kidney using FAS antibody at dilution of 1:400 (200x lens).)

WB (Western Blot)

(Western blot analysis of extracts of SW480 cell lines, using FAS antibody.)

Related Product Information for anti-FAS antibody

Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers (1,2). Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes (3,4). FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-a, TRAIL, and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites (6). FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26 kDa trimeric soluble form (8,9).

Product Categories/Family for anti-FAS antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4507583
• NCBI GeneID: 355
• NCBI Accession #: NP_000034.1
• NCBI GenBank Nucleotide #: NM_000043.4
• UniProt Accession #: P25445; Q14292; Q14293; Q14294; Q14295; Q16652; Q5T9P1; Q5T9P2; Q5T9P3; Q6SSE9; A9UJX4; B6VNV4
• Molecular Weight: 24,781 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 6 isoform 1
• NCBI Official Synonym Full Names: Fas cell surface death receptor
• NCBI Official Symbol: FAS
• NCBI Official Synonym Symbols: APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• UniProt Gene Name: FAS
• UniProt Synonym Gene Names: APT1; FAS1; TNFRSF6
• UniProt Entry Name: TNR6_HUMAN