Rabbit anti-Human FASN Polyclonal Antibody | anti-FASN antibody

FASN Antibody (Center)

Cat. Num. AAA28651

• Gene Names: FASN; FAS; OA-519; SDR27X1
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: FASN, Antibody; FASN Antibody (Center); Fatty acid synthase; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; 3-oxoacyl-[acyl-carrier-protein] synthase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; Enoyl-[acyl-carrier-protein] reductase; Oleoyl-[acyl-carrier-protein] hydrolase; FASN; FAS; anti-FASN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This FASN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 942-973 amino acids from the Central region of human FASN.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 942-973
• Sequence Length: 2511

• Applicable Applications for anti-FASN antibody: Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: IF~~1:10~50; WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM (Flow Cytometry)

(FASN Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry)

(FASN Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FASN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of FASN Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot)

(Western blot analysis of FASN (arrow) using rabbit polyclonal FASN Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FASN gene (Lane 2) (Origene Technologies).)

WB (Western Blot)

(Western blot analysis of FASN Antibody (Center) in Ramos cell line lysates (35ug/lane).FASN (arrow) was detected using the purified Pab.)

IF (Immunofluorescence)

(Fluorescent confocal image of HepG2 cells stained with FASN (Center) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA28651 FASN (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the FASN mainly to the mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000169710).)

Related Product Information for anti-FASN antibody

FASN is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Product Categories/Family for anti-FASN antibody

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction

References

References for protein:
1.Jayakumar A., Tai M.-H.Proc. Natl. Acad. Sci. U.S.A. 92:8695-8699(1995)
2.Kuhajda F.P., Jenner K.Proc. Natl. Acad. Sci. U.S.A. 91:6379-6383(1994)
3.Semenkovich C.F., Coleman T.J. Lipid Res. 36:1507-1521(1995)
References for HepG2 cell line:
1. Knowles BB, et al. (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499.[ PubMed: 6248960].
2. Darlington GJ, et al. (1987). Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354.[PubMed: 3034851].
3. Ihrke, G; Neufeld, EB; Meads, T; Shanks, MR; Cassio, D; Laurent, M; Schroer, TA; Pagano, RE et al. (1993). "WIF-B cells: an in vitro model for studies of hepatocyte polarity". Journal of Cell Biology 123 (6): 1761-1775. [PubMed:7506266].
4. Mersch-Sundermann, V.; Knasmüller, S.; Wu, X. J.; Darroudi, F.; Kassie, F. (2004). "Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents". Toxicology 198 (1-3): 329-340. [PubMed:15138059].

NCBI and Uniprot Product Information

• NCBI GI #: 41872631
• NCBI GeneID: 2194
• NCBI Accession #: NP_004095.4
• NCBI GenBank Nucleotide #: NM_004104.4
• UniProt Accession #: P49327; Q13479; Q16702; Q4LE83; Q6P4U5; Q6SS02; Q969R1; Q96C68; Q96IT0
• Molecular Weight: 273427
• NCBI Official Full Name: fatty acid synthase
• NCBI Official Synonym Full Names: fatty acid synthase
• NCBI Official Symbol: FASN
• NCBI Official Synonym Symbols: FAS; OA-519; SDR27X1
• NCBI Protein Information: fatty acid synthase
• UniProt Protein Name: Fatty acid synthase
• UniProt Gene Name: FASN
• UniProt Synonym Gene Names: FAS
• UniProt Entry Name: FAS_HUMAN