WB (Western Blot)
(Detection of human FBXO11 by western blot. Samples: Whole cell lysate (50 ug) from RKO, K-562, A-549, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FBXO11 antibody (AAA211344 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human FBXO11 Polyclonal Antibody | anti-FBXO11 antibody
Rabbit anti-FBXO11 Antibody, Affinity Purified
Gene Names
FBXO11; UBR6; VIT1; FBX11; PRMT9; UG063H01
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
FBXO11, Antibody; Rabbit anti-FBXO11 Antibody, Affinity Purified; UBR6; vitiligo-associated protein 1; vitiligo-associated protein VIT-1; VIT-1; VIT1; UG063H01; ubiquitin protein ligase E3 component n-recognin 6; protein arginine N-methyltransferase 9; PRMT9; IDDFBA; F-box only protein 11; FBX11; F-box protein 11; anti-FBXO11 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-FBXO11 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat
Immunogen
Between 877 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human FBXO11 by western blot. Samples: Whole cell lysate (50 ug) from RKO, K-562, A-549, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FBXO11 antibody (AAA211344 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human FBXO11 by western blot. Samples: Whole cell lysate (50 ug) from RKO, K-562, A-549, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FBXO11 antibody (AAA211344 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human FBXO11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FBXO11 antibody (AAA211344 lot 2) used for IP at 6 ug per reaction. FBXO11 was also immunoprecipitated by a previous lot of this antibody (AAA211344 lot 1) and a second antibody against a different epitope of FBXO11 . For blotting immunoprecipitated FBXO11, AAA211344 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human FBXO11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FBXO11 antibody (AAA211344 lot 2) used for IP at 6 ug per reaction. FBXO11 was also immunoprecipitated by a previous lot of this antibody (AAA211344 lot 1) and a second antibody against a different epitope of FBXO11 . For blotting immunoprecipitated FBXO11, AAA211344 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-FBXO11 antibody
FBXO11 is a member of the F-box protein family and component of the Skp1-Cullin1-F-box (SCF) ubiquitin ligase complex. FBXO11 has been shown to interact with p53 and suppress its activity. Suppression of p53 activity is achieved via Nedd8 conjugation to p53.
Product Categories/Family for anti-FBXO11 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
103,585 Da
NCBI Official Full Name
F-box only protein 11 isoform 4
NCBI Official Synonym Full Names
F-box protein 11
NCBI Official Symbol
FBXO11
NCBI Official Synonym Symbols
UBR6; VIT1; FBX11; PRMT9; UG063H01
NCBI Protein Information
F-box only protein 11; vitiligo-associated protein 1; vitiligo-associated protein VIT-1; protein arginine N-methyltransferase 9; ubiquitin protein ligase E3 component n-recognin 6
UniProt Protein Name
F-box only protein 11
UniProt Gene Name
FBXO11
UniProt Synonym Gene Names
FBX11; PRMT9; VIT1; VIT-1
UniProt Entry Name
FBX11_HUMAN
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Product Notes
The FBXO11 fbxo11 (Catalog #AAA211344) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-FBXO11 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FBXO11 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the FBXO11 fbxo11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FBXO11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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