Rabbit FGFR4 Polyclonal Antibody | anti-FGFR4 antibody

Phospho-FGFR4 (Tyr754) Antibody

Cat. Num. AAA329717

• Gene Names: FGFR4; TKF; JTK2; CD334
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Zebrafish (86%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: FGFR4, Antibody; Phospho-FGFR4 (Tyr754) Antibody; CD 334; CD334; CD334 antigen; fc13h 10; fc13h10; Fgfr 4; FGFR-4; Fgfr4; FGFR4_HUMAN; Fibroblast growth factor receptor 4; Hydroxyaryl protein kinase; JTK 2; JTK2; MGC20292; Protein tyrosine kinase; TKF; Tyrosine kinase related to fibroblast growth factor receptor; Tyrosylprotein kinase; anti-FGFR4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Zebrafish (86%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-FGFR4 (Tyr754) Antibody detects endogenous levels of FGFR4 only when phosphorylated at Tyr754.; Tissue Specificity: Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-FGFR4 antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human FGFR4 around the phosphorylation site of Tyr754.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: N-glycosylated. Full maturation of the glycan chains in the Golgi is essential for high affinity interaction with FGF19.Ubiquitinated. Subject to proteasomal degradation when not fully glycosylated. Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer.
• Subunit Structure: Monomer. Homodimer after ligand binding. Interacts with FGF1, FGF2, FGF4, FGF6, FGF8, FGF9, FGF16, FGF17, FGF18, FGF19, FGF21 and FGF23 (in vitro). Binding affinity for FGF family members is enhanced by interactions between FGFs and heparan sulfate proteoglycans. Interacts with KLB; this strongly increases the affinity for FGF19 and FGF23. Affinity for FGF19 is strongly increased by KLB and sulfated glycosaminoglycans. KLB and KL both interact with the core-glycosylated FGFR4 in the endoplasmic reticulum and promote its degradation, so that only FGFR4 with fully mature N-glycans is expressed at the cell surface. Identified in a complex with NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Interacts with MMP14 and HIP1. Interacts with STAT3.
• Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
• Subcellular Location: Cell membrane>Single-pass type I membrane protein. Endosome. Endoplasmic reticulum.; Note: Internalized from the cell membrane to recycling endosomes, and from there back to the cell membrane. Secreted. Cytoplasm.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohiostchemistry)

(Staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis FGFR4 (Phospho-Tyr754) using PDGF treated NIH-3T3 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-FGFR4 antibody

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 47524173
• NCBI GeneID: 2264
• NCBI Accession #: NP_002002.3
• NCBI GenBank Nucleotide #: NM_002011.3
• UniProt Accession #: P22455; O43785; Q14309; Q71TW8; Q8TDA0; AAB25788
• Molecular Weight: 87,954 Da
• NCBI Official Full Name: fibroblast growth factor receptor 4 isoform 1
• NCBI Official Synonym Full Names: fibroblast growth factor receptor 4
• NCBI Official Symbol: FGFR4
• NCBI Official Synonym Symbols: TKF; JTK2; CD334
• NCBI Protein Information: fibroblast growth factor receptor 4; FGFR-4; tyrosylprotein kinase; protein-tyrosine kinase; hydroxyaryl-protein kinase; tyrosine kinase related to fibroblast growth factor receptor
• UniProt Protein Name: Fibroblast growth factor receptor 4
• UniProt Gene Name: FGFR4
• UniProt Synonym Gene Names: JTK2; TKF; FGFR-4
• UniProt Entry Name: FGFR4_HUMAN