WB (Western Blot)
(All lanes : Anti-Fgfr4 Antibody (Center) at 1:2000 dilutionLane 1: mouse skeletal muscle lysatesLane 2: mouse spleen lysatesLane 3: rat lung lysatesLane 4: mouse lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Mouse, rat Fgfr4 Polyclonal Antibody | anti-Fgfr4 antibody
Mouse Fgfr4 Antibody (Center)
Gene Names
Fgfr4; Fgfr-4
Reactivity
Mouse, rat
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Fgfr4, Antibody; Mouse Fgfr4 Antibody (Center); Fibroblast growth factor receptor 4; FGFR-4; Protein-tyrosine kinase receptor MPK-11; CD334; Fgfr4; Fgfr-4; Mpk-11; anti-Fgfr4 antibody
Host
Rabbit
Reactivity
Mouse, rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Fgfr4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 448-482 amino acids from the Central region of Mouse Fgfr4.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
799
Applicable Applications for anti-Fgfr4 antibody
ELISA, WB (Western Blot)
Crown Antibody
Yes
Clone Names
RB50681
Antigen Source
HUMAN
Cellular Location
Cell membrane; Single-pass type I membrane protein Endosome. Endoplasmic reticulum Note=Internalized from the cell membrane to recycling endosomes, and from there back to the cell membrane.
Tissue Location
Isoform 1 and isoform 2 are expressed in lung and proliferating myoblasts and myotubes of primary myogenic cells (at protein level). Isoform 1 and isoform 2 are expressed in liver, muscle, spleen, heart, lung, kidney and in primary myogenic cells.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-Fgfr4 Antibody (Center) at 1:2000 dilutionLane 1: mouse skeletal muscle lysatesLane 2: mouse spleen lysatesLane 3: rat lung lysatesLane 4: mouse lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-Fgfr4 Antibody (Center) at 1:2000 dilutionLane 1: mouse skeletal muscle lysatesLane 2: mouse spleen lysatesLane 3: rat lung lysatesLane 4: mouse lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Fgfr4 antibody
Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Plays a role in postnatal lung development. May be involved in the development of skeletal muscle cell lineages.
Product Categories/Family for anti-Fgfr4 antibody
References
Stark K.L.,et al.Development 113:641-651(1991).
Kwiatkowski B.A.,et al.J. Cell. Physiol. 215:803-817(2008).
Carninci P.,et al.Science 309:1559-1563(2005).
Gilardi-Hebenstreit P.,et al.Oncogene 7:2499-2506(1992).
Weinstein M.,et al.Development 125:3615-3623(1998).
Kwiatkowski B.A.,et al.J. Cell. Physiol. 215:803-817(2008).
Carninci P.,et al.Science 309:1559-1563(2005).
Gilardi-Hebenstreit P.,et al.Oncogene 7:2499-2506(1992).
Weinstein M.,et al.Development 125:3615-3623(1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88661
NCBI Official Full Name
fibroblast growth factor receptor 4
NCBI Official Synonym Full Names
fibroblast growth factor receptor 4
NCBI Official Symbol
Fgfr4
NCBI Official Synonym Symbols
Fgfr-4
NCBI Protein Information
fibroblast growth factor receptor 4
UniProt Protein Name
Fibroblast growth factor receptor 4
UniProt Gene Name
Fgfr4
UniProt Synonym Gene Names
Fgfr-4; Mpk-11; FGFR-4
UniProt Entry Name
FGFR4_MOUSE
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Product Notes
The Fgfr4 fgfr4 (Catalog #AAA285836) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Fgfr4 Antibody (Center) reacts with Mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fgfr4 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Fgfr4 fgfr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fgfr4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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