Rabbit FGR Polyclonal Antibody | anti-FGR antibody

Phospho-FGR (Tyr412) Antibody

Cat. Num. AAA329718

• Gene Names: FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (89%), Bovine (89%), Horse (89%), Sheep (89%), Rabbit (89%), Dog (89%)
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: FGR, Antibody; Phospho-FGR (Tyr412) Antibody; c fgr; c fgr protooncogene; c src 2 proto oncogene; c src2; Fgr; FGR_HUMAN; FLJ43153; Gardner Rasheed feline sarcoma viral (v fgr); Gardner Rasheed feline sarcoma viral (v fgr) oncogene homolog; Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog; MGC75096; p55 c fgr protein; P55 FGR; P55-FGR; p55c fgr; p58-Fgr; p58c fgr; p58c-Fgr; Proto oncogene c Fgr; Proto oncogene tyrosine protein kinase FGR; Proto-oncogene c-Fgr; SRC 2; SRC2; Tyrosine protein kinase Fgr; Tyrosine-protein kinase Fgr; anti-FGR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (89%), Bovine (89%), Horse (89%), Sheep (89%), Rabbit (89%), Dog (89%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-FGR (Tyr412) Antibody detects endogenous levels of FGR only when phosphorylated at Tyr412.; Tissue Specificity: Detected in neutrophils, monocytes and natural killer cells (at protein level). Detected in monocytes and large lymphocytes.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-FGR antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human FGR around the phosphorylation site of Tyr412.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Ubiquitinated. Becomes ubiquitinated in response to ITGB2 signaling; this does not lead to degradation.Phosphorylated. Autophosphorylated on tyrosine residues. Becomes phosphorylated in response to FCGR2A and/or FCGR2B engagement, cell adhesion and signaling by ITGB2. Prior phosphorylation at Tyr-523 by SRC inhibits ulterior autophosphorylation at Tyr-412.
• Subunit Structure: Interacts with ITGB1, ITGB2, MS4A2/FCER1B, FCER1G, FCGR2A and/or FCGR2B. Interacts (via SH2 domain) with SYK (tyrosine phosphorylated). Interacts (via SH2 domain) with FLT3 (tyrosine phosphorylated). Interacts with PTK2/FAK1. Interacts (via SH2 domain) with HCLS1 (tyrosine phosphorylated by SYK). Interacts with SIRPA and PTPNS1. Interacts (not phosphorylated on tyrosine residues) with CBL; FGR tyrosine phosphorylation promotes dissociation. Interacts with PIK3R1 and FASLG (By similarity). Interacts with CLNK (By similarity).
• Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
• Subcellular Location: Cell membrane>Lipid-anchor>Cytoplasmic side. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Ruffle membrane. Cytoplasm>Cytosol. Cytoplasm>Cytoskeleton. Mitochondrion inner membrane. Mitochondrion intermembrane space.; Note: Detected in mitochondrial intermembrane space and at inner membranes (By similarity). Colocalizes with actin fibers at membrane ruffles. Detected at plasma membrane lipid rafts.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemistry)

(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemisry)

(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohiostchemistry)

(Staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis FGR (Phospho-Tyr412) using Mouse brain tissue lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-FGR antibody

Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1. Together with CLNK, it acts as a negative regulator of natural killer cell-activating receptors and inhibits interferon-gamma production (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 112382241
• NCBI GeneID: 2268
• NCBI Accession #: NP_001036194.1
• NCBI GenBank Nucleotide #: NM_001042729.1
• UniProt Accession #: P09769; Q9UIQ3; D3DPL7
• Molecular Weight: 59,479 Da
• NCBI Official Full Name: tyrosine-protein kinase Fgr
• NCBI Official Synonym Full Names: Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
• NCBI Official Symbol: FGR
• NCBI Official Synonym Symbols: SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
• NCBI Protein Information: tyrosine-protein kinase Fgr; p55-c-fgr protein; c-fgr protooncogene; proto-oncogene c-Fgr; c-src-2 proto-oncogene; proto-oncogene tyrosine-protein kinase FGR
• UniProt Protein Name: Tyrosine-protein kinase Fgr
• UniProt Gene Name: FGR
• UniProt Synonym Gene Names: SRC2
• UniProt Entry Name: FGR_HUMAN