IP (Immunoprecipitation)
(Detection of human FIH-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-FIH-1 antibody AAA212220 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated FIH-1, rabbit anti-FIH-1 antibody BL12623, which recognizes and upstream epitope, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human FIH-1 Polyclonal Antibody | anti-FIH-1 antibody
Rabbit anti-FIH-1 Antibody, Affinity Purified
Gene Names
HIF1AN; FIH1
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
FIH-1, Antibody; Rabbit anti-FIH-1 Antibody, Affinity Purified; factor inhibiting HIF-1; peptide-aspartate beta-dioxygenase; hypoxia-inducible factor asparagine hydroxylase; hypoxia-inducible factor 1-alpha inhibitor; hypoxia inducible factor 1 alpha subunit inhibitor; FIH1; factor inhibiting HIF1; FIH-1; Hypoxia-inducible factor 1-alpha inhibitor; hypoxia inducible factor 1 subunit alpha inhibitor; anti-FIH-1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-FIH-1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Mouse, Zebrafish
Immunogen
Between 299 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human FIH-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-FIH-1 antibody AAA212220 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated FIH-1, rabbit anti-FIH-1 antibody BL12623, which recognizes and upstream epitope, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human FIH-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-FIH-1 antibody AAA212220 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated FIH-1, rabbit anti-FIH-1 antibody BL12623, which recognizes and upstream epitope, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human FIH-1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-FIH-1 (Cat. No. AAA212220 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human FIH-1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-FIH-1 (Cat. No. AAA212220 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
Related Product Information for anti-FIH-1 antibody
Factor inhibiting HIF-1 (FIH-1) hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). FIH-1 functions as an oxygen sensor, and under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. FIH-1 is involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NWT6].
Product Categories/Family for anti-FIH-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,285 Da
NCBI Official Full Name
hypoxia-inducible factor 1-alpha inhibitor
NCBI Official Synonym Full Names
hypoxia inducible factor 1, alpha subunit inhibitor
NCBI Official Symbol
HIF1AN
NCBI Official Synonym Symbols
FIH1
NCBI Protein Information
hypoxia-inducible factor 1-alpha inhibitor; FIH-1; factor inhibiting HIF-1; factor inhibiting HIF1; hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase
UniProt Protein Name
Hypoxia-inducible factor 1-alpha inhibitor
UniProt Gene Name
HIF1AN
UniProt Synonym Gene Names
FIH1; FIH-1
UniProt Entry Name
HIF1N_HUMAN
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Product Notes
The FIH-1 hif1an (Catalog #AAA212220) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-FIH-1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FIH-1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the FIH-1 hif1an for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FIH-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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