WB (Western Blot)
(Detection of human FIP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FIP1 antibody AAA211484 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. FIP1 was also immunoprecipitated by rabbit anti-FIP1 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).)
Rabbit anti-Human FIP1 Polyclonal Antibody | anti-FIP1 antibody
Rabbit anti-FIP1 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
FIP1, Antibody; Rabbit anti-FIP1 Antibody, Affinity Purified; FIP1 like 1; Rhe; rearranged in hypereosinophilia; pre-mRNA 3'-end-processing factor FIP1; hFip1; FIP1L1 cleavage and polyadenylation specific factor subunit; FIP1; factor interacting with PAP; FIP1-like 1 protein; Pre-mRNA 3'-end-processing factor FIP1; factor interacting with PAPOLA and CPSF1; anti-FIP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-FIP1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human FIP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FIP1 antibody AAA211484 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. FIP1 was also immunoprecipitated by rabbit anti-FIP1 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).)
WB (Western Blot)
(Detection of human FIP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FIP1 antibody AAA211484 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. FIP1 was also immunoprecipitated by rabbit anti-FIP1 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).)
IHC (Immunohistochemistry)
(Detection of human FIP1 by immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti-FIP1 (Cat. No. AAA211484) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human FIP1 by immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti-FIP1 (Cat. No. AAA211484) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-FIP1 antibody
FIP1 was originally identified as Rhe (rearranged in hypereosinophilia) and part of a fusion protein encoded by a genetic rearrangement between the N-terminal region of FIP1 (Rhe) and the C-terminal region of the platelet-derived growth factor receptor alpha (PDGFRalpha). The fusion protein exhibits aberrant tyrosine kinase activity and is associated with hypereosinophilic syndrome (HES), a rare hematologic disorder characterized by an overproduction of eosinophils that results in organ damage. FIP1 was also identified as FIP1-like 1 in a database search for a human homolog of the yeast Fip1p (factor interacting with PAP), a subunit of the yeast 3'-end processing complex, CPF (cleavage and polyadenylation factor). Human FIP1 was similarly found to interact with PAP and CPSF160 as part of the human CPSF (cleavage and polyadenylation specificity factor) complex. In association with CPSF160, FIP1 is able to recognize U-rich elements on pre-mRNA and stimulate PAP activity.
Product Categories/Family for anti-FIP1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The FIP1 fip1l1 (Catalog #AAA211484) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-FIP1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FIP1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the FIP1 fip1l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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