WB (Western Blot)
(Detection of human FIP200/RB1CC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, RKO, U2OS, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FIP200/RB1CC1 antibody (AAA211522 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse FIP200/RB1CC1 Polyclonal Antibody | anti-FIP200/RB1CC1 antibody
Rabbit anti-FIP200/RB1CC1 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
FIP200/RB1CC1, Antibody; Rabbit anti-FIP200/RB1CC1 Antibody, Affinity Purified; ATG17; PPP1R131; RB1-inducible coiled-coil protein 1; phosphatase 1, regulatory subunit 131; CC1; 200 kDa FAK family kinase-interacting protein; FAK family kinase-interacting protein of 200 kDa; FIP200; RB1 inducible coiled-coil 1; anti-FIP200/RB1CC1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-FIP200/RB1CC1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1544 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human FIP200/RB1CC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, RKO, U2OS, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FIP200/RB1CC1 antibody (AAA211522 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human FIP200/RB1CC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, RKO, U2OS, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FIP200/RB1CC1 antibody (AAA211522 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse FIP200/RB1CC1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FIP200/RB1CC1 antibody (AAA211522 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of mouse FIP200/RB1CC1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FIP200/RB1CC1 antibody (AAA211522 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human FIP200/RB1CC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FIP200/RB1CC1 antibody (AAA211522 lot 2) used for IP at 6 ug per reaction. FIP200/RB1CC1 was also immunoprecipitated by a previous lot of this antibody (AAA211522 lot 1) and a second antibody against a different epitope of FIP200/RB1CC1 . For blotting immunoprecipitated FIP200/RB1CC1, AAA211522 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human FIP200/RB1CC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FIP200/RB1CC1 antibody (AAA211522 lot 2) used for IP at 6 ug per reaction. FIP200/RB1CC1 was also immunoprecipitated by a previous lot of this antibody (AAA211522 lot 1) and a second antibody against a different epitope of FIP200/RB1CC1 . For blotting immunoprecipitated FIP200/RB1CC1, AAA211522 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-FIP200/RB1CC1 antibody
FIP200 (FAK-family interacting protein of 200 kDa) appears to play several key roles related to cell growth, proliferation, development, and tumorigenesis. FIP200 was originally identified in a yeast two-hybrid screen for Pyk2 kinase interacting proteins. It was later identified as a protein that interacts with FAK (focal adhesion kinase), a signal transducing protein that mediates signals from the extracellular matrix to the cytoskeleton to influence cell motility, and cell cycle progression. FIP200 is also known as RB1CC1 (RB1-inducible coiled-coil 1) and has been found to function as a regulator of the retinoblastoma 1 (RB1) tumor suppressor. FIP200 associates with the tumor suppressor TSC1 which partners with TSC2 to antagonize the mTOR signaling pathway. FIP200 association with TSC2 promotes mTOR activity by interfering with the antagonistic effects of the TSC1-TSC2 complex.
Product Categories/Family for anti-FIP200/RB1CC1 antibody
NCBI and Uniprot Product Information
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Product Notes
The FIP200/RB1CC1 rb1cc1 (Catalog #AAA211522) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-FIP200/RB1CC1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FIP200/RB1CC1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the FIP200/RB1CC1 rb1cc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FIP200/RB1CC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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