IF (Immunofluorescence)
(AAA328925 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328925) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Rabbit FKBP51 Polyclonal Antibody | anti-FKBP5 antibody
FKBP51 Antibody
Gene Names
FKBP5; P54; AIG6; FKBP51; FKBP54; PPIase; Ptg-10
Reactivity
Human, Mouse, RatPredicted Reactivity: Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
FKBP51, Antibody; FKBP51 Antibody; 51 kDa FK506 binding protein 5; 51 kDa FK506 binding protein; 51 kDa FK506-binding protein; 51 kDa FKBP; 54 kDa progesterone receptor associated immunophilin; 54 kDa progesterone receptor-associated immunophilin; AIG 6; AIG6; Androgen regulated protein 6; Androgen-regulated protein 6; FF1 antigen; FK506 binding protein 5; FK506-binding protein 5; FKBP 5; FKBP 51; FKBP 54; FKBP-5; FKBP-51; FKBP5; FKBP5_HUMAN; FKBP54; HSP90 binding immunophilin; HSP90-binding immunophilin; MGC111006; OTTHUMP00000016268; P54; Peptidyl prolyl cis trans isomerase; Peptidyl-prolyl cis-trans isomerase FKBP5; Peptidylprolyl cis trans isomerase; PPIase; PPIase FKBP5; Ptg 10; Ptg10; Rotamase; T cell FK506 binding protein; anti-FKBP5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
FKBP51 Antibody detects endogenous levels of total FKBP51.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
457
Applicable Applications for anti-FKBP5 antibody
ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human FKBP51, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328925 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328925) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
IF (Immunofluorescence)
(AAA328925 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328925) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using FKBP51 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using FKBP51 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-FKBP5 antibody
Function: Immunophilin protein with PPIase and co-chaperone activities (PubMed:11350175). Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded (PubMed:12538866). Acts as a regulator of Akt/AKT1 activity by promoting the interaction between Akt/AKT1 and PHLPP1, thereby enhancing dephosphorylation and subsequent activation of Akt/AKT1 (PubMed:28147277).
Post Translational Modifications: Acetylation impairs ability to promote interaction between Akt/AKT1 and PHLPP1 (PubMed:28147277). Deacetylation by SIRT7 promotes interaction between Akt/AKT1 and PHLPP1, leading to suppress Akt/AKT1 activation (PubMed:28147277).
Subcellular Location: Cytoplasm. Nucleus.
Tissue Specificity: Widely expressed, enriched in testis compared to other tissues.
Subunit Structure: Part of a heteromultimeric cytoplasmic complex with HSP90AA1, HSPA1A/HSPA1B and steroid receptors. Upon ligand binding dissociates from the complex and FKBP4 takes its place (By similarity). Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP (PubMed:7693698). Interacts with NR3C1 (By similarity). Interacts with Akt/AKT1 and PHLPP1; enhancing dephosphorylation and subsequent activation of Akt/AKT1 (PubMed:28147277).
Post Translational Modifications: Acetylation impairs ability to promote interaction between Akt/AKT1 and PHLPP1 (PubMed:28147277). Deacetylation by SIRT7 promotes interaction between Akt/AKT1 and PHLPP1, leading to suppress Akt/AKT1 activation (PubMed:28147277).
Subcellular Location: Cytoplasm. Nucleus.
Tissue Specificity: Widely expressed, enriched in testis compared to other tissues.
Subunit Structure: Part of a heteromultimeric cytoplasmic complex with HSP90AA1, HSPA1A/HSPA1B and steroid receptors. Upon ligand binding dissociates from the complex and FKBP4 takes its place (By similarity). Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP (PubMed:7693698). Interacts with NR3C1 (By similarity). Interacts with Akt/AKT1 and PHLPP1; enhancing dephosphorylation and subsequent activation of Akt/AKT1 (PubMed:28147277).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)51kD.
Predicted Molecular Weight: (Calculated)51kDa.
Predicted Molecular Weight: (Calculated)51kDa.
NCBI Official Full Name
Peptidyl-prolyl cis-trans isomerase FKBP5
NCBI Official Synonym Full Names
FKBP prolyl isomerase 5
NCBI Official Symbol
FKBP5
NCBI Official Synonym Symbols
P54; AIG6; FKBP51; FKBP54; PPIase; Ptg-10
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase FKBP5
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase FKBP5
UniProt Gene Name
FKBP5
UniProt Synonym Gene Names
AIG6; FKBP51; PPIase FKBP5; 51 kDa FKBP; FKBP-51; FKBP-5; p54
UniProt Entry Name
FKBP5_HUMAN
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Product Notes
The FKBP5 fkbp5 (Catalog #AAA328925) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FKBP51 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's FKBP51 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the FKBP5 fkbp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FKBP51, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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