WB (Western Blot)
(Detection of human FOX2/RBM9 by western blot. Samples: Whole cell lysate (10 ug) from U2OS, HEK293T, Hep-G2, HeLa, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM9 antibody (AAA23776 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit FOX2/RBM9 Polyclonal Antibody | anti-FOX2/RBM9 antibody
Rabbit anti-FOX2/RBM9 Antibody, Affinity Purified
IP: 6ug/mg lysate
IHC: 1:2000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 ul per 1 x 10^6 cells.
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human FOX2/RBM9 by western blot. Samples: Whole cell lysate (10 ug) from U2OS, HEK293T, Hep-G2, HeLa, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM9 antibody (AAA23776 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human FOX2/RBM9 by western blot. Samples: Whole cell lysate (10 ug) from U2OS, HEK293T, Hep-G2, HeLa, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM9 antibody (AAA23776 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human FOX2/RBM9 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, TCMK-1, CT26, and C6 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM9 antibody AAA23776 (lot AAA23776-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human FOX2/RBM9 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, TCMK-1, CT26, and C6 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM9 antibody AAA23776 (lot AAA23776-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human FOX2/RBM9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RBM9 antibody (AAA23776 lot 4) used for IP at 6 ug per reaction. RBM9 was also immunoprecipitated by a previous lot of this antibody (AAA23776 lot 3) and a second antibody to a different epitope of RBM9 (BL2440). For blotting immunoprecipitated RBM9, AAA23776 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human FOX2/RBM9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RBM9 antibody (AAA23776 lot 4) used for IP at 6 ug per reaction. RBM9 was also immunoprecipitated by a previous lot of this antibody (AAA23776 lot 3) and a second antibody to a different epitope of RBM9 (BL2440). For blotting immunoprecipitated RBM9, AAA23776 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohistochemistry)
(Detection of human FOX2/RBM9 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RBM9 (AAA23776 Lot 4) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human FOX2/RBM9 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RBM9 (AAA23776 Lot 4) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
FCM (Flow Cytometry)
(Detection of human RBM9 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-RBM9 polyclonal antibody (AAA23776)) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human RBM9 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-RBM9 polyclonal antibody (AAA23776)) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of mouse RBM9 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-RBM9 polyclonal antibody (AAA23776)) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of mouse RBM9 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-RBM9 polyclonal antibody (AAA23776)) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
NCBI and Uniprot Product Information
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Product Notes
The FOX2/RBM9 rbfox2 (Catalog #AAA23776) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-FOX2/RBM9 Antibody, Affinity Purified reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FOX2/RBM9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:2000-1:10,000 IP: 6ug/mg lysate IHC: 1:2000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the FOX2/RBM9 rbfox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOX2/RBM9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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